Antibacterial activity evaluation of quaternary chitin against Escherichia coli and Staphylococcus aureus

被引:37
作者
Huang, Jianying [1 ]
Jiang, Hengjun [1 ]
Qiu, Miao [1 ]
Geng, Xiaodong [1 ]
Yang, Ronghua [1 ]
Li, Jianrong [2 ]
Zhang, Chaohua [3 ]
机构
[1] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China
[2] Bohai Univ, Coll Chem Chem Engn & Food Safety, Jinzhou 121003, Peoples R China
[3] Guangdong Ocean Univ, Zhanjiang 524088, Guangdong, Peoples R China
基金
美国国家科学基金会;
关键词
Water-soluble; Chitin; Chitosan; Antibacterial activity; CHITOSAN; CHLORIDE; SALT;
D O I
10.1016/j.ijbiomac.2012.10.017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Water-soluble quaternary chitin/chitosan derivatives, O-[(2-hydroxy-3-trimethylammonium)propyl chitin (OHT-chitin), N-[(2-hydroxy-3-trimethylammonium)propyl chitosan (NHT-chitosan), and N,N,N-trimethylchitosan (TMC), having identical molecular weight and same anion, were prepared, and their antibacterial activities against Escherichia coli and Staphylococcus aureus were evaluated. Their minimum inhibitory concentration (MIC) values varied from 0.04 mg/mL to 20.48 mg/mL, and the minimum bactericidal concentration (MBC) values varied from 0.16 mg/mL to 40.96 mg/mL against E. coli and S. aureus, respectively. Transmission electron microscopy (TEM) clearly showed that there was serious damage on the bacterial walls, whereas there was some dissimilarity between TMC-treated E. coli and S. aureus. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:85 / 91
页数:7
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