Electrochemical multianalyte immunoassays using an array-based sensor

被引:134
作者
Wilson, MS [1 ]
Nie, WY [1 ]
机构
[1] EIC Labs Inc, Norwood, MA 02062 USA
关键词
D O I
10.1021/ac0518452
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel amperometric biosensor for performing simultaneous electrochemical multianalyte immunoassays is described. The sensor consisted of eight iridium oxide sensing electrodes (0.78 mm(2) each), an iridium counter electrode, and a Ag/AgCl reference electrode patterned on a glass substrate. Four different capture antibodies were immobilized on the sensing electrodes via adsorption. Quantification of proteins was achieved using an ELISA in which the electrochemical oxidation of enzyme-generated hydroquinone was measured. The spatial separation of the electrodes enabled simultaneous electrochemical immunoassays for multiple proteins to be conducted in a single assay without amperometric crosstalk between the electrodes. The simultaneous detection of goat IgG, mouse IgG, human IgG, and chicken IgY was demonstrated. The detection limit was 3 ng/mL for all analytes. The sensor had excellent precision (1.9-8.2% interassay CV) and was comparable in performance to commercial single-analyte ELISAs. We anticipate that chip-based sensors, as described herein, will be suitable for the mass production of economical, miniaturized, multianalyte assay devices.
引用
收藏
页码:2507 / 2513
页数:7
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