Kinetic behavior of phosphotriesterase and its non-covalent complexes with polyelectrolyte in systems with polar and non-polar organic solvents

被引：17

作者：

Sergeeva, VS ^{[1
]}

Efremenko, EN ^{[1
]}

Kazankov, GM ^{[1
]}

Gladilin, AK ^{[1
]}

Varfolomeev, SD ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Chem Enzymol Div, Moscow 119899, Russia

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 07期

关键词：

immobilized cells; organic solvent; phosphotriesterase; polyelectrolyte; stabilization;

D O I：

10.1023/A:1008985602921

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Soluble phosphotriesterase from E. coli DH5 alpha together with E. coli DH5 alpha cells with the phosphotriesterase activity were co-immobilized into poly(vinyl alcohol) (PVA) cryogel and studied in water/organic systems with polar and non-polar organic solvents. The phosphotriesterase activity was competitively inhibited by polar organic solvents. The inhibition constant correlated with the dielectric constant (epsilon) of the solvent. The rate of the enzyme-catalyzed reaction in biphasic non-polar solvent/water systems was independent of water/organic ratio and the hydrophobicity of the solvent. Formation of the non-covalent complexes with polyelectrolytes was suggested to enhance the resistance of the phosphotriesterase towards inactivation by organic solvents in their homogeneous mixtures with water.

引用

页码：479 / 483

页数：5

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