RNA-Based Fluorescent Biosensors for Live Cell Imaging of Second Messengers Cyclic di-GMP and Cyclic AMP-GMP

被引：194

作者：

Kellenberger, Colleen A. ^{[1
]}

Wilson, Stephen C. ^{[1
]}

Sales-Lee, Jade ^{[1
]}

Hammond, Ming C. ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 13期

关键词：

ESCHERICHIA-COLI; LIGAND-BINDING; RIBOSWITCH; DIGUANYLATE; MAGNESIUM; BACTERIA; CGMP; DNA;

D O I：

10.1021/ja311960g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are lacking. We have designed RNA-based fluorescent biosensors for cyclic di-GMP and cyclic AMP-GMP by fusing the Spinach aptamer to variants of a natural GEMM-1 riboswitch. In live cell imaging experiments, these biosensors demonstrate fluorescence turn-on in response to cyclic dinucleotides, and they were used to confirm in vivo production of cyclic AMP-GMP by the enzyme DncV.

引用

页码：4906 / 4909

页数：4

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