MOLECULAR CHARACTERIZATION OF SOME STRAINS OF NEWCASTLE DISEASE VIRUS ISOLATED IN PROVINCE OF VOJVODINA, REPUBLIC OF SERBIA

被引：0
|
作者：
Milic, N. [1 ]
Lazic, S. [2 ]
Vidanovic, D. [3 ]
Sekler, M. [3 ]
Nisavic, J.
Radmila, Resanovic
Petrovic, T. [2 ]
机构：
[1] Univ Belgrade, Fac Vet Med, Dept Microbiol, Belgrade 11000, Serbia
[2] Sci Vet Inst Novi Sad, Novi Sad, Serbia
[3] Vet Specialized Inst Kraljevo, Kraljevo, Serbia
来源：
ACTA VETERINARIA-BEOGRAD | 2012年 / 62卷 / 04期
关键词：
Newcastle disease virus; phylogenetic analysis; RT-PCR; GENOTYPE; OUTBREAKS; FUSION; SITE;
D O I：
10.2298/AVB1204365M
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Five strains of Newcastle disease virus (NDV) were obtained from poultry in Vojvodina, Serbia during the outbreaks in 2006 and 2007. These isolates were confirmed and genotypically characterized by reverse transcription polymerase chain reaction (RT-PCR) with primer specific to the viral fusion (F) protein (572bp), and by sequencing of partial F gene for phylogenetic analysis. Phylogenetic analysis showed that all five isolated strains of Newcastle disease virus belong to genotype VII. At the same time, all five isolates were clustered in NDV subtype VIId. The examined NOV isolates express high similarity to each other (99.7-100%) and group together with the strains of Newcastle disease virus isolated previously from wild birds in Serbia during the same 2006 - 2007 outbreak. The analysis of the isolates F gene cleavage sites has shown that all five isolated strains of Newcastle disease virus had a cleavage site motif 112R-R-Q-K-R-F-117 characteristic for highly virulent, velogenic strains.
引用
收藏
页码：365 / 374
页数：10
相关论文
共 50 条
  • [1] Characterization of Newcastle Disease Virus Isolated from Waterfowl in China
    Liu, Hualei
    Wang, Zhiliang
    Wang, Yongkun
    Sun, Chengying
    Zheng, Dongxia
    Wu, Yangong
    AVIAN DISEASES, 2008, 52 (01) : 150 - 155
  • [2] GENETIC CHARACTERIZATION OF EGYPTIAN NEWCASTLE DISEASE VIRUS STRAINS ISOLATED FROM FLOCKS VACCINATED AGAINST NEWCASTLE DISEASE VIRUS, 2014-2015
    Megahed, Mohamed M.
    Eid, Amal A. M.
    Mohamed, Walaa
    Hassanin, Ola
    SLOVENIAN VETERINARY RESEARCH, 2018, 55 : 17 - 29
  • [3] Pathotypic characterization of the Newcastle disease virus isolated from commercial poultry in northwest Iran
    Ahmadi, Elham
    Pourbakhsh, Seyed Ali
    Ahmadi, Malahat
    Talebi, Alireza
    TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, 2014, 38 (04): : 383 - 387
  • [4] The comparative antigenic characterization of Newcastle disease virus strains isolated in Kenya and Kazakhstan
    Panshin, A
    Shihmanter, E
    Weisman, Y
    Örvell, C
    Kydyrmanov, A
    Sayatov, M
    Asanov, N
    Nyaga, PN
    Kasiiti, JL
    Macharia, MJ
    Lipkind, M
    COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2001, 24 (01) : 21 - 37
  • [5] Molecular and biological characterization of some circulating strains of Newcastle disease virus in broiler chickens from Eastern Saudi Arabia in 2012-2014
    Almubarak, Abdullah I. A.
    VETERINARY WORLD, 2019, 12 (10) : 1668 - 1676
  • [6] Molecular epidemiological analysis of Newcastle disease virus isolated in China in 2005
    Liu, Hualei
    Wang, Zhiliang
    Wu, Yangong
    Zheng, Dongxia
    Sun, Chengying
    Bi, Daorong
    Zuo, Yuanyuan
    Xu, Tiangang
    JOURNAL OF VIROLOGICAL METHODS, 2007, 140 (1-2) : 206 - 211
  • [7] Molecular characterization of a virulent strain of Newcastle disease virus isolated from a diseased chicken in Kyrgyzstan in 2016
    Giang Tran Thi Huong
    Hieu Dong Van
    Tung Dao Duy
    Iskender, Saadanov
    Mairambek, Isakeev
    Omatsu, Tsutomu
    Katayama, Yukie
    Mizutani, Tetsuya
    Ozeki, Yuki
    Takeda, Yohei
    Ogawa, Haruko
    Imai, Kunitoshi
    JAPANESE JOURNAL OF VETERINARY RESEARCH, 2019, 67 (04) : 263 - 273
  • [8] Molecular and phylogenetic characterization based on the complete genome of a virulent pathotype of Newcastle disease virus isolated in the 1970s in Brazil
    Fernandes, Camila C.
    Varani, Alessandro M.
    Lemos, Eliana G. M.
    de Miranda, Vitor Fernandes O.
    Silva, Ketherson R.
    Fernando, Filipe S.
    Montassier, Maria F. S.
    Montassier, Helio J.
    INFECTION GENETICS AND EVOLUTION, 2014, 26 : 160 - 167
  • [9] Molecular Characterization of Thermostable Newcastle disease virus Isolated from Pigeon
    Uthrakumar, A.
    Vijayarani, K.
    Kumanan, K.
    Thiagarajan, V.
    INDIAN JOURNAL OF VIROLOGY, 2013, 24 (01): : 42 - 47
  • [10] Characterization of genotype IX Newcastle disease virus strains isolated from wild birds in the northern Qinling Mountains, China
    Duan, Xuji
    Zhang, Peng
    Ma, Jing
    Chen, Shengli
    Hao, Huafang
    Liu, Haijin
    Fu, Xiangjing
    Wu, Pengpeng
    Zhang, Dingquan
    Zhang, Weidong
    Du, Enqi
    Yang, Zengqi
    VIRUS GENES, 2014, 48 (01) : 48 - 55
12345