Overexpression of Holocarboxylase Synthetase Predicts Lymph Node Metastasis and Unfavorable Prognosis in Breast Cancer

被引：10

作者：

Sukjoi, Witchuda ^{[1
]}

Siritutsoontorn, Siraprapa ^{[1
]}

Chansongkrow, Pakkanan ^{[1
]}

Waiwitlikhit, Suppakit ^{[1
,2
]}

Polyak, Steven W. ^{[3
]}

Warnnissorn, Malee ^{[4
]}

Charoensawan, Varodom ^{[1
,5
,6
]}

Thuwajit, Chanitra ^{[7
]}

Jitrapakdee, Sarawut ^{[1
]}

机构：

[1] Mahidol Univ, Dept Biochem, Fac Sci, Rama 6 Rd, Bangkok 10400, Thailand

[2] Brighton Coll, Brighton, E Sussex, England

[3] Univ South Australia, Clin & Hlth Sci, Adelaide, SA, Australia

[4] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Pathol, Bangkok, Thailand

[5] Mahidol Univ, Fac Sci, Syst Biol Dis Res Unit, Bangkok, Thailand

[6] Mahidol Univ, Integrat Computat BioSci ICBS Ctr, Nakhon Pathom, Thailand

[7] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Immunol, Bangkok, Thailand

来源：

ANTICANCER RESEARCH | 2020年 / 40卷 / 08期

基金：

英国医学研究理事会;

关键词：

Holocarboxylase synthetase; biotin-dependent carboxylases; biotin; breast cancer; metabolism; ACID SYNTHESIS PATHWAY; PYRUVATE-CARBOXYLASE; METABOLISM; PROTEIN; GROWTH; CELLS;

D O I：

10.21873/anticanres.14461

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Holocarboxylase synthetase (HLCS) catalyzes the specific attachment of biotin onto biotin-dependent carboxylases (BDCs) which play important roles in intermediary metabolism. Previous studies show that BDCs are overexpressed in many cancer types. However, expression of HLCS in cancerous tissues has not been reported. Materials and Methods: Immunohistochemistry was used to investigate HLCS expression in breast tissue obtained from 65 Thai patients, and the correlation between its expression and key clinical-pathological parameters was assessed. The role of HLCS in supporting invasion was investigated in HLCS-knockdown MCF-7 cells. Results: Overexpression of HLCS was significantly associated with metastasis of breast cancer cells to other lymph nodes but not the sentinel and axillary lymph nodes - a finding supported in cellular invasion assays using HLCS knockdown cells. Furthermore, overexpression of HLCS reduced survival time of patients with breast cancer. Conclusion: HLCS appears to be a prognostic marker for patients with breast cancer.

引用

页码：4557 / 4565

页数：9