Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1

被引：82

作者：

Gorny, Miroslaw K. ^{[1
]}

Wang, Xiao-Hong ^{[3
]}

Williams, Constance ^{[1
]}

Volsky, Barbara ^{[1
]}

Revesz, Kathy ^{[3
]}

Witover, Bradley ^{[1
]}

Burda, Sherri ^{[1
]}

Urbanski, Mateusz ^{[1
]}

Nyambi, Phillipe ^{[1
,3
]}

Krachmarov, Chavdar ^{[4
]}

Pinter, Abraham ^{[4
]}

Zolla-Pazner, Susan ^{[1
,3
]}

Nadas, Arthur ^{[2
]}

机构：

[1] NYU, Sch Med, Dept Pathol, New York, NY 10016 USA

[2] NYU, Sch Med, Inst Environm Med, New York, NY 10016 USA

[3] Vet Affairs New York Harbor Healthcare Syst, New York, NY 10010 USA

[4] Publ Hlth Res Inst, Newark, NJ 07103 USA

来源：

MOLECULAR IMMUNOLOGY | 2009年 / 46卷 / 05期

关键词：

Immunoglobulin gene usage; Human monoclonal antibodies; Anti-HIV-1; antibodies; HUMAN-IMMUNODEFICIENCY-VIRUS; HUMAN MONOCLONAL-ANTIBODIES; EXTENSIVE SOMATIC MUTATION; POLYMERASE-CHAIN-REACTION; EQUATORIAL RAIN-FORESTS; MEMORY B-CELLS; MOLECULAR CHARACTERIZATION; TYPE-1; GP120; CORECEPTOR SELECTIVITY; ENVELOPE GLYCOPROTEIN;

D O I：

10.1016/j.molimm.2008.09.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human anti-V3 monoclonal antibodies (mAbs) generated from HIV-1 infected individuals display diversity in the range of their cross-neutralization that may be related to their immunogenetic background. The study of the immunoglobulin (Ig) variable region gene usage of heavy chains have shown a preferential usage of the VH5-51 gene segment which was detected in 35% of 51 human anti-V3 mAbs. In contrast, human mAbs against other envelope regions of HIV-1 (anti-Env), including the CD4-binding domain, the CD4-induced epitope, and gp41 preferentially used the VH1-69 gene segment, and none of them used the VH5-51 gene, Furthermore, the usage of the VH4 family by anti-V3 mAbs was restricted to only one gene segment, VH4-59, while the VH3 gene family was used at a significantly lower frequency by all of the analyzed anti-HIV-1 mAbs. Multivariate analysis showed that usage of VH gene segments was significantly different between anti-V3 and anti-Env mAbs, and compared to antibodies from healthy subjects. In addition, the anti-V3 mAbs preferentially used the JH3 and D2-15 gene segments. The preferential usage of selected Ig gene segments and the characteristic pattern of Ig gene usage by anti-V3 mAbs can be related to the conserved structure of the V3 region. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：917 / 926

页数：10

共 62 条

[1] MOLECULAR CHARACTERIZATION OF 5 HUMAN ANTI-HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ANTIBODY HEAVY-CHAINS REVEALS EXTENSIVE SOMATIC MUTATION TYPICAL OF AN ANTIGEN-DRIVEN IMMUNE-RESPONSE [J].