Preferential use of the VH5-51 gene segment by the human immune response to code for antibodies against the V3 domain of HIV-1
被引:83
作者:
Gorny, Miroslaw K.
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Gorny, Miroslaw K.
[1
]
Wang, Xiao-Hong
论文数: 0引用数: 0
h-index: 0
机构:
Vet Affairs New York Harbor Healthcare Syst, New York, NY 10010 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Wang, Xiao-Hong
[3
]
Williams, Constance
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Williams, Constance
[1
]
Volsky, Barbara
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Volsky, Barbara
[1
]
Revesz, Kathy
论文数: 0引用数: 0
h-index: 0
机构:
Vet Affairs New York Harbor Healthcare Syst, New York, NY 10010 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Revesz, Kathy
[3
]
Witover, Bradley
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Witover, Bradley
[1
]
Burda, Sherri
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Burda, Sherri
[1
]
Urbanski, Mateusz
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Urbanski, Mateusz
[1
]
Nyambi, Phillipe
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Vet Affairs New York Harbor Healthcare Syst, New York, NY 10010 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Nyambi, Phillipe
[1
,3
]
Krachmarov, Chavdar
论文数: 0引用数: 0
h-index: 0
机构:
Publ Hlth Res Inst, Newark, NJ 07103 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Krachmarov, Chavdar
[4
]
Pinter, Abraham
论文数: 0引用数: 0
h-index: 0
机构:
Publ Hlth Res Inst, Newark, NJ 07103 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Pinter, Abraham
[4
]
Zolla-Pazner, Susan
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Vet Affairs New York Harbor Healthcare Syst, New York, NY 10010 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Zolla-Pazner, Susan
[1
,3
]
Nadas, Arthur
论文数: 0引用数: 0
h-index: 0
机构:
NYU, Sch Med, Inst Environm Med, New York, NY 10016 USANYU, Sch Med, Dept Pathol, New York, NY 10016 USA
Nadas, Arthur
[2
]
机构:
[1] NYU, Sch Med, Dept Pathol, New York, NY 10016 USA
[2] NYU, Sch Med, Inst Environm Med, New York, NY 10016 USA
[3] Vet Affairs New York Harbor Healthcare Syst, New York, NY 10010 USA
Human anti-V3 monoclonal antibodies (mAbs) generated from HIV-1 infected individuals display diversity in the range of their cross-neutralization that may be related to their immunogenetic background. The study of the immunoglobulin (Ig) variable region gene usage of heavy chains have shown a preferential usage of the VH5-51 gene segment which was detected in 35% of 51 human anti-V3 mAbs. In contrast, human mAbs against other envelope regions of HIV-1 (anti-Env), including the CD4-binding domain, the CD4-induced epitope, and gp41 preferentially used the VH1-69 gene segment, and none of them used the VH5-51 gene, Furthermore, the usage of the VH4 family by anti-V3 mAbs was restricted to only one gene segment, VH4-59, while the VH3 gene family was used at a significantly lower frequency by all of the analyzed anti-HIV-1 mAbs. Multivariate analysis showed that usage of VH gene segments was significantly different between anti-V3 and anti-Env mAbs, and compared to antibodies from healthy subjects. In addition, the anti-V3 mAbs preferentially used the JH3 and D2-15 gene segments. The preferential usage of selected Ig gene segments and the characteristic pattern of Ig gene usage by anti-V3 mAbs can be related to the conserved structure of the V3 region. (C) 2008 Elsevier Ltd. All rights reserved.