Mechanical Strain Determines Cilia Length, Motility, and Planar Position in the Left-Right Organizer

被引:23
|
作者
Chien, Yuan-Hung [1 ]
Srinivasan, Shyam [1 ,2 ]
Keller, Ray [3 ]
Kintner, Chris [1 ]
机构
[1] Salk Inst Biol Studies, Mol Neurobiol Lab, La Jolla, CA 92037 USA
[2] Univ Calif San Diego, Kavil Inst Brain & Mind, La Jolla, CA 92093 USA
[3] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA
关键词
LEFT-RIGHT SYMMETRY; XENOPUS-LAEVIS; CELL POLARITY; PROSPECTIVE AREAS; FLUID-FLOW; NODE; SPECIFICATION; GASTRULATION; EXPRESSION; MOVEMENTS;
D O I
10.1016/j.devcel.2018.04.007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The Xenopus left-right organizer (LRO) breaks symmetry along the left-right axis of the early embryo by producing and sensing directed ciliary flow as a patterning cue. To carry out this process, the LRO contains different ciliated cell types that vary in cilia length, whether they are motile or sensory, and how they position their cilia along the anterior-posterior (A-P) planar axis. Here, we show that these different cilia features are specified in the prospective LRO during gastrulation, based on anisotropic mechanical strain that is oriented along the A-P axis, and graded in levels along the medial-lateral axis. Strain instructs ciliated cell differentiation by acting on a mesodermal prepattern present at blastula stages, involving foxjl. We propose that differential strain is a graded, developmental cue, linking the establishment of an A-P planar axis to cilia length, motility, and planar location during formation of the Xenopus LRO.
引用
收藏
页码:316 / +
页数:19
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