Online LC-GC Analysis of Free Sterols/Stanols and Intact Steryl/Stanyl Esters in Cereals

被引：22
作者：
Esche, Rebecca [1 ]
Scholz, Birgit [1 ]
Engel, Karl-Heinz [1 ]
机构：
[1] Tech Univ Munich, Lehrstuhl Allgemeine Lebensmitteltechnol, D-85350 Freising Weihenstephan, Germany
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2013年 / 61卷 / 46期
关键词：
free sterols/stanols; steryl/stanyl esters; sweet corn; popcorn; proso millet; online LC-GC; CHROMATOGRAPHY-GAS CHROMATOGRAPHY; ORYZA-SATIVA L; EDIBLE OILS; GAMMA-ORYZANOL; PANICUM-MILIACEUM; STEROLS; LIPIDS; CORN; PHYTOSTEROLS; INTERFACE;
D O I：
10.1021/jf403046z
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The suitability of online liquid chromatography gas chromatography for the analysis of free sterols/stanols, steryl/stanyl fatty acid esters, and trans-steryl/stanyl ferulic acid esters in cereals is demonstrated. The silylated lipid extracts were fractionated via liquid chromatography on a normal phase, and the fractions containing the sterol classes were transferred online to the gas chromatograph for the analysis of their individual compositions. The study provides for the first time data on free sterols/stanols and intact steryl/stanyl esters in sweet corn, popcorn, and proso millet. Sweet corn revealed the highest contents of free sterols/stanols and steryl/stanyl fatty acid esters, and popcorn, in turn, the highest amounts of trans-steryl/stanyl ferulic acid esters. The distribution patterns of the proso millet samples revealed pronounced differences from those of sweet corn and popcorn as they particularly exhibited high proportions of free cholesterol and cholesteryl fatty acid esters. Furthermore, no trans-steryl/stanyl ferulic acid esters could be detected.
引用
收藏
页码：10932 / 10939
页数：8
相关论文
共 33 条
  • [1] [Anonymous], 2008, DIN 32645:2008-11
  • [2] ONLINE LC-GC FOR THE ANALYSIS OF THE MINOR COMPONENTS IN EDIBLE OILS AND FATS - THE DIRECT METHOD INVOLVING SILYLATION
    ARTHO, A
    GROB, K
    MARIANI, C
    [J]. FETT WISSENSCHAFT TECHNOLOGIE-FAT SCIENCE TECHNOLOGY, 1993, 95 (05): : 176 - 180
  • [3] PROTEIN CHARACTERIZATION AND NUTRIENT COMPOSITION OF HUNGARIAN PROSO MILLET VARIETIES AND THE EFFECT OF DECORTICATION
    Bagdi, A.
    Balazs, G.
    Schmidt, J.
    Szatmari, M.
    Schoenlechner, R.
    Berghofer, E.
    Toemoeskoezia, S.
    [J]. ACTA ALIMENTARIA, 2011, 40 (01) : 128 - 141
  • [4] Bamsteiner A., 2012, FOOD CONTROL, V27, P275
  • [5] Bamsteiner A., 2011, J AGR FOOD CHEM, V59, P5204
  • [6] Quantitative analyses of individual γ-Oryzanol (Steryl Ferulates) in conventional and organic brown rice (Oryza sativa L.)
    Cho, Jeong-Yong
    Lee, Hyoung Jae
    Kim, Gee An
    Kim, Gwi Deok
    Lee, You Seok
    Shin, Soo Cheol
    Park, Keun-Hyung
    Moon, Jae-Hak
    [J]. JOURNAL OF CEREAL SCIENCE, 2012, 55 (03) : 337 - 343
  • [7] Analysis of free phytosterols/stanols and their intact fatty acid and phenolic acid esters in various corn cultivars
    Esche, Rebecca
    Scholz, Birgit
    Engel, Karl-Heinz
    [J]. JOURNAL OF CEREAL SCIENCE, 2013, 58 (02) : 333 - 340
  • [8] Analysis of phytosteryl and phytostanyl fatty acid esters in enriched dairy foods: a combination of acid digestion, lipid extraction, and on-line LC-GC
    Esche, Rebecca
    Carcelli, Alessandro
    Barnsteiner, Andreas
    Sforza, Stefano
    Engel, Karl-Heinz
    [J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2013, 236 (06) : 999 - 1007
  • [9] Simultaneous Analysis of Free Phytosterols/Phytostanols and Intact Phytosteryl/Phytostanyl Fatty Acid and Phenolic Acid Esters in Cereals
    Esche, Rebecca
    Barnsteiner, Andreas
    Scholz, Birgit
    Engel, Karl-Heinz
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (21) : 5330 - 5339
  • [10] Grob K., 1989, Journal of High Resolution Chromatography and Chromatography Communications, V12, P624, DOI 10.1002/jhrc.1240120913
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