Pumpkin (Cucurbita ficifolia Bouche) extract attenuate the adipogenesis in human mesenchymal stem cells by controlling adipogenic gene expression

被引:12
作者
Alshammari, Ghedeir M. [1 ]
Balakrishnan, Aristatile [1 ]
机构
[1] King Saud Univ, Coll Food & Agr Sci, Dept Food Sci & Nutr, Adipocytes & Metab Disorders Lab, POB 2460, Riyadh 11451, Saudi Arabia
关键词
Obesity; Cucurbita ficifolia; Adipogenesis; Human mesenchymal stem cells (hMSCs); ADIPOSE-TISSUE; BONE-MARROW; SEED OIL; OBESITY; INSULIN; DIFFERENTIATION; GAMMA; FAT; INFLAMMATION; METABOLISM;
D O I
10.1016/j.sjbs.2018.10.002
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Prevention and management of obesity through dietary modification is one of the top way to trim down its consequences. Development of adipose tissue requires the differentiation of less specialized cells, such as human mesenchymal stem cells (hMSCs), into adipocytes. Since food constituents play a major role in the cell differentiation and proliferation, we sought to determine if various extracts of Cucurbita ficifolia (C. ficifolia), could affect the adipogenic differentiation of hMSCs. Flow cytometry analysis with quantitative and qualitative Nile red, and quantitative PCR methods were employed to evaluate the C. ficifolia effect on hMSCs adipogenesis. Results revealed that, chloroform extract exhibits significant adipogenic inhibition than that of hexane and methanol extracts. Chloroform extract treated cells display the down-regulation of ADIPOQ, FABP4, PPARGC1A, CEBPB & LPL and up-regulation of ACACB & CEBPA genes. Further, various phytoconstituents present in the chloroform extract of C. ficifolia were analyzed though LC-MS and GC-MS. Our results indicates that chloroform extract of C. ficifolia might be used as a food supplement to control obesity and its related consequences. (C) 2018 Production and hosting by Elsevier B.V. on behalf of King Saud University.
引用
收藏
页码:744 / 751
页数:8
相关论文
共 40 条
[1]   Adipose tissue as an endocrine organ [J].
Ahima, RS ;
Flier, JS .
TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2000, 11 (08) :327-332
[2]  
Akhtar M S, 1982, J Pak Med Assoc, V32, P106
[3]   Pumpkin-seed oil modulates the effect of felodipine and captopril in spontaneously hypertensive rats [J].
Al Zuhair, H ;
El-Fattah, AAA ;
El-Sayed, MI .
PHARMACOLOGICAL RESEARCH, 2000, 41 (05) :555-563
[4]   CUDC-907 Promotes Bone Marrow Adipocytic Differentiation Through Inhibition of Histone Deacetylase and Regulation of Cell Cycle [J].
Ali, Dalia ;
Alshammari, Hassan ;
Vishnubalaji, Radhakrishnan ;
Chalisserry, Elna Paul ;
Hamam, Rimi ;
Alfayez, Musaad ;
Kassem, Moustapha ;
Aldahmash, Abdullah ;
Alajez, Nehad M. .
STEM CELLS AND DEVELOPMENT, 2017, 26 (05) :353-362
[5]   Efficacy of simvastatin and pumpkin-seed oil in the management of dietary-induced hypercholesterolemia [J].
AlZuhair, H ;
AbdElFattah, AA ;
AbdElLatif, HA .
PHARMACOLOGICAL RESEARCH, 1997, 35 (05) :403-408
[6]   In vitro biocompatibility and proliferative effects of polar and non-polar extracts of cucurbita ficifolia on human mesenchymal stem cells [J].
Aristatile, Balakrishnan ;
Alshammari, Ghedeir M. .
BIOMEDICINE & PHARMACOTHERAPY, 2017, 89 :215-220
[7]   Epidemiology of Obesity and Diabetes and Their Cardiovascular Complications [J].
Bhupathiraju, Shilpa N. ;
Hu, Frank B. .
CIRCULATION RESEARCH, 2016, 118 (11) :1723-1735
[8]   Dietary fat intake does affect obesity! [J].
Bray, GA ;
Popkin, BM .
AMERICAN JOURNAL OF CLINICAL NUTRITION, 1998, 68 (06) :1157-1173
[9]   A water-soluble extract from Cucurbita moschata shows anti-obesity effects by controlling lipid metabolism in a high fat diet-induced obesity mouse model [J].
Choi, Hyounjeong ;
Eo, Haekwan ;
Park, Kyoungcheol ;
Jin, Mirim ;
Park, Eun-Jin ;
Kim, Seon-Hee ;
Park, Jeong Euy ;
Kim, Sunyoung .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2007, 359 (03) :419-425
[10]   The role of C/EBP genes in adipocyte differentiation [J].
Darlington, GJ ;
Ross, SE ;
MacDougald, OA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (46) :30057-30060