Whole-genome sequencing disproves two suspected transmission events of blaNDM between Pseudomonas aeruginosa and Enterobacterales in hospitalized patients

被引：0

作者：

Kocer, K. ^{[1
]}

Boutin, S. ^{[1
,2
,3
]}

Probst, K. ^{[1
]}

Heeg, K. ^{[1
]}

Nurjadi, D. ^{[1
]}

机构：

[1] Heidelberg Univ Hosp, Dept Infect Dis Med Microbiol & Hyg, Neuenheimer Feld 324, D-69120 Heidelberg, Germany

[2] Heidelberg Univ Hosp, Translat Lung Res Ctr Heidelberg, Heidelberg, Germany

[3] Heidelberg Univ Hosp, German Ctr Lung Res, Heidelberg, Germany

来源：

JOURNAL OF HOSPITAL INFECTION | 2020年 / 106卷 / 02期

关键词：

Whole-genome sequencing; bla(NDM); Horizonal gene transfer; Multi-drug-resistant organism; Plasmid; Outbreak management;

D O I：

10.1016/j.jhin.2020.07.006

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

New Delhi metallo-beta-lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of bIaNDM between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different bIaNDM variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks. (C) 2020 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

引用

收藏

页码：372 / 375

页数：4

相关论文

共 10 条

[1] Challenges in interpretation of WGS and epidemiological data to investigate nosocomial transmission of vancomycin-resistant Enterococcus faecium in an endemic region: incorporation of patient movement network and admission screening [J].

Eichel, Vanessa ;

Klein, Sabrina ;

Bootsveld, Carolin ;

Frank, Uwe ;

Heeg, Klaus ;

Boutin, Sebastien ;

Nurjadi, Dennis .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2020, 75 (07) :1716-1721

[2] Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health [J].

Khan, Asad U. ;

Maryam, Lubna ;

Zarrilli, Raffaele .

BMC MICROBIOLOGY, 2017, 17

[3]

KOCER M, 2019, CLIN MICROBIOL INFEC, V26, DOI DOI 10.1016/J.JOBE.2019.100878

[4] Acquired qnrVC1 and bla NDM-1 resistance markers in an international high-risk Pseudomonas aeruginosa ST773 clone [J].

Kocsis, Bela ;

Toth, Akos ;

Gulyas, Daniel ;

Ligeti, Balazs ;

Katona, Katalin ;

Rokusz, Laszlo ;

Szabo, Dora .

JOURNAL OF MEDICAL MICROBIOLOGY, 2019, 68 (03) :336-338

[5] The emerging NDM carbapenemases [J].

Nordmann, Patrice ;

Poirel, Laurent ;

Walsh, Timothy R. ;

Livermore, David M. .

TRENDS IN MICROBIOLOGY, 2011, 19 (12) :588-595

[6] Whole genome sequencing options for bacterial strain typing and epidemiologic analysis based on single nucleotide polymorphism versus gene-by-gene-based approaches [J].

Schurch, A. C. ;

Arredondo-Alonso, S. ;

Willems, R. J. L. ;

Goering, R. V. .

CLINICAL MICROBIOLOGY AND INFECTION, 2018, 24 (04) :350-354

[7] TRANSFER OF PLASMIDS FROM ESCHERICHIA-COLI TO PSEUDOMONAS-AERUGINOSA - CHARACTERIZATION OF A PSEUDOMONAS-AERUGINOSA MUTANT WITH ENHANCED RECIPIENT ABILITY FOR ENTEROBACTERIAL PLASMIDS [J].

TARDIF, G ;

GRANT, RB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1983, 24 (02) :201-208

[8] IS26-Mediated Transfer of blaNDM-1 as the Main Route of Resistance Transmission During a Polyclonal, Multispecies Outbreak in a German Hospital [J].

Weber, Robert E. ;

Pietsch, Michael ;

Fruehauf, Andre ;

Pfeifer, Yvonne ;

Martin, Maria ;

Luft, Dirk ;

Gatermann, Soeren ;

Pfennigwerth, Niels ;

Kaase, Martin ;

Werner, Guido ;

Fuchs, Stephan .

FRONTIERS IN MICROBIOLOGY, 2019, 10

[9] NDM Metallo-β-Lactamases and Their Bacterial Producers in Health Care Settings [J].

Wu, Wenjing ;

Feng, Yu ;

Tang, Guangmin ;

Qiao, Fu ;

McNally, Alan ;

Zong, Zhiyong .

CLINICAL MICROBIOLOGY REVIEWS, 2019, 32 (02)

[10] A multiresistant clone of Pseudomonas aeruginosa sequence type 773 spreading in a burn unit in Orumieh, Iran [J].

Yousefi, Saber ;

Nahaei, Mohammad Reza ;

Farajnia, Safar ;

Aghazadeh, Mohammad ;

Iversen, Aina ;

Edquist, Petra ;

Maatallah, Makaoui ;

Giske, Christian G. .

APMIS, 2013, 121 (02) :146-152