Non-viral gene delivery systems

被引:363
作者
Davis, ME [1 ]
机构
[1] CALTECH, Pasadena, CA 91125 USA
关键词
D O I
10.1016/S0958-1669(02)00294-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Non-viral gene delivery systems have the potential to create viable pharmaceuticals from nucleic acids. These DNA delivery systems contain lipids and/or cationic polymers. In order for these systems to be developed into commercial products, several barriers must be overcome. These include obstacles in manufacturing, formulation and stability. In vivo, problems of extracellular non-specific interactions and intracellular trafficking to the nucleus are also encountered. Recent progress has been made in overcoming these issues.
引用
收藏
页码:128 / 131
页数:4
相关论文
共 35 条
[1]   Stabilization of lipid/DNA complexes during the freezing step of the lyophilization process: the particle isolation hypothesis [J].
Allison, SD ;
Molina, MDC ;
Anchordoquy, TJ .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1468 (1-2) :127-138
[2]   Physical stabilization of DNA-based therapeutics [J].
Anchordoquy, TJ ;
Allison, SD ;
Molina, MDC ;
Girouard, LG ;
Carson, TK .
DRUG DISCOVERY TODAY, 2001, 6 (09) :463-470
[3]  
BANASZCZYK MG, 1999, PURE APPL CHEM, V36, P1061
[4]   Different strategies for formation of PEGylated EGF-conjugated PEI/DNA complexes for targeted gene delivery [J].
Blessing, T ;
Kursa, M ;
Holzhauser, R ;
Kircheis, R ;
Wagner, E .
BIOCONJUGATE CHEMISTRY, 2001, 12 (04) :529-537
[5]   Overcoming the nuclear barrier:: Cell cycle independent nonviral gene transfer with linear polyethylenimine or electroporation [J].
Brunner, S ;
Fürtbauer, E ;
Sauer, T ;
Kursa, M ;
Wagner, E .
MOLECULAR THERAPY, 2002, 5 (01) :80-86
[6]   Cell cycle dependence of gene transfer by lipoplex polyplex and recombinant adenovirus [J].
Brunner, S ;
Sauer, T ;
Carotta, S ;
Cotten, M ;
Saltik, M ;
Wagner, E .
GENE THERAPY, 2000, 7 (05) :401-407
[7]   A pH-sensitive polymer that enhances cationic lipid-mediated gene transfer [J].
Cheung, CY ;
Murthy, N ;
Stayton, PS ;
Hoffman, AS .
BIOCONJUGATE CHEMISTRY, 2001, 12 (06) :906-910
[8]  
Collard WT, 2000, J PHARM SCI-US, V89, P499
[9]   Decreased binding to proteins and cells of polymeric gene delivery vectors surface modified with a multivalent hydrophilic polymer and retargeting through attachment of transferrin [J].
Dash, PR ;
Read, ML ;
Fisher, KD ;
Howard, KA ;
Wolfert, M ;
Oupicky, D ;
Subr, V ;
Strohalm, J ;
Ulbrich, K ;
Seymour, LW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (06) :3793-3802
[10]  
DAVIS ME, 2000, Patent No. 00033885