Recombinant Haemonchus contortus 24 kDa excretory/secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro

被引：28
作者：
Gadahi, Javaid Ali [1 ,2 ]
Li, Baojie [1 ]
Ehsan, Muhammad [1 ]
Wang, Shuai [1 ]
Zhang, Zhenchao [1 ]
Wang, Yujian [1 ]
Hasan, Muhammad Waqqas [1 ]
Yan, Ruofeng [1 ]
Song, Xiaokai [1 ]
Xu, Lixin [1 ]
Li, Xiangrui [1 ]
机构：
[1] Nanjing Agr Univ, Coll Vet Med, Nanjing, Jiangsu, Peoples R China
[2] Sindh Agr Univ Tandojam, Dept Vet Parasitol, Tandojam, Pakistan
来源：
ONCOTARGET | 2016年 / 7卷 / 51期
关键词：
H; contortus; ESP; 24; kDa; cytokines; proliferation; cell migration; Immunology and Microbiology Section; Immune response; Immunity; ANCYLOSTOMA-SECRETED PROTEIN; REGULATORY T-CELLS; NITRIC-OXIDE; MOLECULAR CHARACTERIZATION; NECATOR-AMERICANUS; HOOKWORM; NEMATODE; PARASITE; INFECTION; RESPONSES;
D O I：
10.18632/oncotarget.13487
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
A 24 kDa protein is one of the important components in Haemonchus contortus (barber pole worm) excretory/secretory products (HcESPs), which was shown to have important antigenic function. However, little is known about the immunomodulatory effects of this protein on host cell. In the present study gene encoding 24kDa excretory/secretory protein (HcES-24) was cloned. The recombinant protein of HcES-24 (rHcES-24) was expressed in a histidine-tagged fusion protein soluble form in Escherichia coli. Binding activity of rHcES-24 to goat PBMCs was confirmed by immunofluorescence assay (IFA) and its immunomudulatory effect on cytokine secretion, cell proliferation, cell migration and nitric oxide production were observed by co-incubation of rHcES-24. IFA results revealed that rHcES-24 could bind to the PBMCs. The interaction of rHcES-24 increased the production of IL4, IL10, IL17 and cell migration in dose dependent manner. However, rHcES-24 treatment significantly suppressed the production of IFN gamma, proliferation of the PBMC and Nitric oxide (NO) production. Our findings showed that the rHcES-24 played important regulatory effects on the goat PBMCs.
引用
收藏
页码：83926 / 83937
页数：12
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