A review on detection methods used for foodborne pathogens

被引:131
作者
Priyanka, B. [1 ]
Patil, Rajashekhar K. [1 ]
Dwarakanath, Sulatha [2 ]
机构
[1] Mangalore Univ, Dept Appl Zool, Mangaluru, India
[2] Nanosci Diagnost, Austin, TX 78726 USA
关键词
Biosensors; culture based; diagnostics; ELISA; foodborne pathogens; PCR; ESCHERICHIA-COLI O157-H7; REVERSE-TRANSCRIPTASE PCR; IN-GROUND BEEF; REAL-TIME; MULTIPLEX PCR; RAPID DETECTION; IMMUNOMAGNETIC SEPARATION; SALMONELLA-TYPHIMURIUM; CHROMAGAR STEC; LABEL-FREE;
D O I
10.4103/0971-5916.198677
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Foodborne pathogens have been a cause of a large number of diseases worldwide and more so in developing countries. This has a major economic impact. It is important to contain them, and to do so, early detection is very crucial. Detection and diagnostics relied on culture-based methods to begin with and have developed in the recent past parallel to the developments towards immunological methods such as enzyme-linked immunosorbent assays (ELISA) and molecular biology-based methods such as polymerase chain reaction (PCR). The aim has always been to find a rapid, sensitive, specific and costeffective method. Ranging from culturing of microbes to the futuristic biosensor technology, the methods have had this common goal. This review summarizes the recent trends and brings together methods that have been developed over the years.
引用
收藏
页码:327 / 338
页数:12
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