Association between BRAF (V600E) mutation and clinicopathological features of papillary thyroid carcinoma: a Brazilian single-centre case series

被引:21
作者
Pessoa-Pereira, Danielle [1 ]
da Silva Medeiros, Mateus Fernandes [1 ]
Sampaio Lima, Virna Mendonca [1 ]
da Silva Jr, Joaquim Custodio [1 ]
de Oliveira Cerqueira, Taise Lima [1 ]
da Silva, Igor Campos [2 ]
Fonseca Jr, Luciano Espinheira [2 ,3 ]
Lobao Sampaio, Luiz Jose [4 ]
Alves de Limas, Claudio Rogerio [5 ]
Ramos, Helton Estrela [1 ]
机构
[1] Univ Fed Bahia UFBA, Inst Saude & Ciencia, Dept Biorregulacao, Lab Estudo Tireoide, Salvador, BA, Brazil
[2] Hosp Sao Rafael, Dept Anat Patol & Citopatol, Salvador, BA, Brazil
[3] Univ Fed Bahia UFBA, Fac Med Bahia, Dept Anat Patol & Med Legal, Salvador, BA, Brazil
[4] Hosp Sao Rafael, Dept Med Nucl, Salvador, BA, Brazil
[5] Hosp Sao Rafael, Dept Cirurgia Cabeca & Pescoco, Salvador, BA, Brazil
来源
ARCHIVES OF ENDOCRINOLOGY METABOLISM | 2019年 / 63卷 / 02期
关键词
Papillary thyroid cancer; BRAF mutation; Hashimoto's thyroiditis; MANAGEMENT GUIDELINES; PROGNOSTIC-FACTORS; CANCER INCIDENCE; HIGH PREVALENCE; NODULES; EXPRESSION; RISK; INFLAMMATION; MORTALITY; PROGRAM;
D O I
10.20945/2359-3997000000120
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objectives: We aimed to investigate the prevalence of the BRAF (V600E) mutation in consecutive cases of papillary thyroid carcinoma (PTC) in patients diagnosed and treated at the Hospital Sao Rafael (Salvador, BA, Brazil) and evaluate its association with clinical and pathological characteristics of PTC. Subjects and methods: We retrospectively enrolled in the study a total of 43 consecutive PTC patients who underwent total thyroidectomy. We performed DNA extraction from formalin-fixed paraffin-embedded (FFPE) tumour tissue samples. Polymerase chain reaction (PCR) and direct sequencing were used to determine BRAF (V600E) mutation status. Univariate and multivariate logistic regression analyses were employed to identify independent associations. Results: The prevalence of BRAF (V600E) mutation was 65.1% (28/43). A high frequency of older patients (p value: 0.004) was observed among the BRAF-mutated PTC group and, in contrast, a low frequency of concurrent Hashimoto's thyroiditis (HT) (p value: 0.011) was noted. Multivariate analysis confirmed that older age (OR: 1.15; 95% CI: 1.00 - 1.33; p value: 0.047) and HT (OR: 0.05; 95% CI: 0.006-0.40; p value: 0.005) were independent factors associated with BRAF (V600E) mutation. Conclusion: We found a high prevalence of BRAF (V600E) mutation in PTC cases. Older age and no concurrent HT were independently associated with BRAF (V600E) mutation.
引用
收藏
页码:97 / 106
页数:10
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