Ganoderic Acid Me Inhibits Tumor Invasion Through Down-Regulating Matrix Metalloproteinases 2/9 Gene Expression

被引：95

作者：

Chen, Nian-Hong ^{[2
]}

Liu, Jian-Wen ^{[2
]}

Zhong, Jian-Jiang ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Key Lab Microbial Metab, Minist Educ, Coll Life Sci & Biotechnol, Shanghai 200240, Peoples R China

[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2008年 / 108卷 / 02期

关键词：

ganoderic acid Me (GA-Me); invasion; matrix metalloproteinases 2/9 (MMP2/9);

D O I：

10.1254/jphs.SC0080019

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205-210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for anti-metastasis treatment of carcinoma cells.

引用

页码：212 / 216

页数：5

共 15 条

[1] Anti-invasive gene expression profile of curcumin in lung adenocarcinoma based on a high throughput microarray analysis [J].