Generation of two RNF2 homozygous knockout human embryonic stem cell lines by CRISPR/Cas9 system

被引：0

作者：

Shan, Yongli ^{[1
,2
]}

Ma, Lishi ^{[1
]}

Zhang, Cong ^{[2
]}

Zhang, Yanqi ^{[2
]}

Zhang, Jingyuan ^{[2
]}

Xiao, Lu ^{[1
]}

Wei, Yanxing ^{[1
]}

Yu, Yanhong ^{[1
]}

机构：

[1] Southern Med Univ, Nanfang Hosp, Dept Obstet & Gynecol, Placenta Res Grp, Guangzhou 510515, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, South China Inst Stem Cell Biol & Regenerat Med, CAS Key Lab Regenerat Biol, Guangzhou 510530, Peoples R China

来源：

STEM CELL RESEARCH | 2020年 / 47卷

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

D O I：

10.1016/j.scr.2020.101885

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human RNF2 (RING1B) gene is a critical epigenetic modification factor for embryonic development, pluripotency and differentiation of embryonic stem cells (ESCs). To further gain insights into the role of RNF2 in cell fate decisions of human ESCs, here we generated two RNF2 homozygous knockout human ESC lines by CRISPR/Cas9 genome editing technology. These cell lines maintained a normal karyotype and typical undifferentiated state in terms of morphology, pluripotent gene expression, and had differentiation potential in vivo. These cell lines provide good cell resources to explore the role of RNF2 gene in embryonic development and lineage differentiation in vitro.

引用

页数：4

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