Lactate Stimulates a Potential for Hypertrophy and Regeneration of Mouse Skeletal Muscle

被引:43
作者
Ohno, Yoshitaka [1 ]
Ando, Koki [1 ]
Ito, Takafumi [1 ]
Suda, Yohei [1 ]
Matsui, Yuki [1 ]
Oyama, Akiko [1 ]
Kaneko, Hikari [1 ]
Yokoyama, Shingo [1 ]
Egawa, Tatsuro [2 ]
Goto, Katsumasa [1 ,3 ]
机构
[1] Toyohashi SOZO Univ, Sch Hlth Sci, Lab Physiol, Toyohashi, Aichi 4408511, Japan
[2] Kyoto Univ, Grad Sch Human & Environm Studies, Lab Sports & Exercise Med, Kyoto 6068501, Japan
[3] Toyohashi SOZO Univ, Grad Sch Hlth Sci, Dept Physiol, Toyohashi, Aichi 4408511, Japan
关键词
lactate; skeletal muscle; hypertrophy; regeneration; muscle satellite cell; SATELLITE CELL; RESISTANCE EXERCISE; DIFFERENTIATION;
D O I
10.3390/nu11040869
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The effects of lactate on muscle mass and regeneration were investigated using mouse skeletal muscle tissue and cultured C2C12 cells. Male C57BL/6J mice were randomly divided into (1) control, (2) lactate (1 mol/L in distilled water, 8.9 mL/g body weight)-administered, (3) cardio toxin (CTX)-injected (CX), and (4) lactate-administered after CTX-injection (LX) groups. CTX was injected into right tibialis anterior (TA) muscle before the oral administration of sodium lactate (five days/week for two weeks) to the mice. Oral lactate administration increased the muscle weight and fiber cross-sectional area, and the population of Pax7-positive nuclei in mouse TA skeletal muscle. Oral administration of lactate also facilitated the recovery process of CTX-associated injured mouse TA muscle mass accompanied with a transient increase in the population of Pax7-positive nuclei. Mouse myoblast-derived C2C12 cells were differentiated for five days to form myotubes with or without lactate administration. C2C12 myotube formation with an increase in protein content, fiber diameter, length, and myo-nuclei was stimulated by lactate. These observations suggest that lactate may be a potential molecule to stimulate muscle hypertrophy and regeneration of mouse skeletal muscle via the activation of muscle satellite cells.
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页数:10
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