The radical SAM enzyme spore photoproduct lyase employs a tyrosyl radical for DNA repair

被引：18

作者：

Kneuttinger, Andrea Christa ^{[1
]}

Heil, Korbinian ^{[1
]}

Kashiwazaki, Gengo ^{[1
]}

Carell, Thomas ^{[1
]}

机构：

[1] Univ Munich, Ctr Integrat Prot Sci, Dept Chem, D-81377 Munich, Germany

来源：

CHEMICAL COMMUNICATIONS | 2013年 / 49卷 / 07期

关键词：

BACILLUS-SUBTILIS; MECHANISM; DAMAGE; INSIGHTS; SPL;

D O I：

10.1039/c2cc37735g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The spore photoproduct lyase is a radical SAM enzyme, which repairs 5-(alpha-thyminyl)-5,6-dihydrothymidine. Here we show that the enzyme establishes a complex radical transfer cascade and creates a cysteine and a tyrosyl radical dyade to establish repair. This allows the enzyme to solve topological and energetic problems associated with the radical based repair reaction.

引用

页码：722 / 724

页数：3

共 19 条

[1] Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme [J].

Benjdia, Alhosna ;

Heil, Korbinian ;

Barends, Thomas R. M. ;

Carell, Thomas ;

Schlichting, Ilme .

NUCLEIC ACIDS RESEARCH, 2012, 40 (18) :9308-9318

[2] Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily [J].

Buis, Jeffrey M. ;

Cheek, Jennifer ;

Kalliri, Efthalia ;

Broderick, Joan B. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (36) :25994-26003

[3] Ultraviolet radiation-mediated damage to cellular DNA [J].

Cadet, J ;

Sage, E ;

Douki, T .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2005, 571 (1-2) :3-17

[4] DNA Repair and Free Radicals, New Insights into the Mechanism of Spore Photoproduct Lyase Revealed by Single Amino Acid Substitution [J].

Chandor-Proust, Alexia ;

Berteau, Olivier ;

Douki, Thierry ;

Gasparutto, Didier ;

Ollagnier-de-Choudens, Sandrine ;

Fontecave, Marc ;

Atta, Mohamed .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (52) :36361-36368

[5] Direct H atom abstraction from spore photoproduct C-6 initiates DNA repair in the reaction catalyzed by spore photoproduct lyase: Evidence for a reversibly generated adenosyl radical intermediate [J].

Cheek, J ;

Broderick, JB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (12) :2860-2861

[6] Spore Photoproduct: A Key to Bacterial Eternal Life [J].

Desnous, Celine ;

Guillaume, Dominique ;

Clivio, Pascale .

CHEMICAL REVIEWS, 2010, 110 (03) :1213-1232

[7] Effects of the binding of α/β-type small, acid-soluble spore proteins on the photochemistry of DNA in spores of Bacillus subtilis and in vitro [J].

Douki, T ;

Setlow, B ;

Setlow, P .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2005, 81 (01) :163-169

[8] MOLECULAR-CLONING AND CHARACTERIZATION OF THE BACILLUS-SUBTILIS SPORE PHOTOPRODUCT LYASE (SPL) GENE, WHICH IS INVOLVED IN REPAIR OF UV RADIATION-INDUCED DNA DAMAGE DURING SPORE GERMINATION [J].

FAJARDOCAVAZOS, P ;

SALAZAR, C ;

NICHOLSON, WL .

JOURNAL OF BACTERIOLOGY, 1993, 175 (06) :1735-1744

[9] Crystal Structures and Repair Studies Reveal the Identity and the Base-Pairing Properties of the UV-Induced Spore Photoproduct DNA Lesion [J].

Heil, Korbinian ;

Kneuttinger, Andrea Christa ;

Schneider, Sabine ;

Lischke, Ulrike ;

Carell, Thomas .

CHEMISTRY-A EUROPEAN JOURNAL, 2011, 17 (35) :9651-9657

[10] Chemical investigation of light induced DNA bipyrimidine damage and repair [J].

Heil, Korbinian ;

Pearson, David ;

Carell, Thomas .

CHEMICAL SOCIETY REVIEWS, 2011, 40 (08) :4271-4278

← 1 2 →