Universal light-sheet generation with field synthesis

被引：65

作者：

Chang, Bo-Jui ^{[1
]}

Kittisopikul, Mark ^{[2
,3
]}

Dean, Kevin M. ^{[1
]}

Roudot, Philippe ^{[1
,4
]}

Welf, Erik S. ^{[1
,4
]}

Fiolka, Reto ^{[1
,4
]}

机构：

[1] UT Southwestern Med Ctr, Dept Cell Biol, Dallas, TX 75390 USA

[2] UT Southwestern Med Ctr, Dept Biophys, Dallas, TX USA

[3] Northwestern Univ, Feinberg Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA

[4] UT Southwestern Med Ctr, Lyda Hill Dept Bioinformat, Dallas, TX 75390 USA

来源：

NATURE METHODS | 2019年 / 16卷 / 03期

基金：

美国国家卫生研究院;

关键词：

FLUORESCENCE MICROSCOPY; ILLUMINATION MICROSCOPY; EXCITATION; CELLS; VIEW;

D O I：

10.1038/s41592-019-0327-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.

引用

页码：235 / +

页数：6

共 24 条

[1] Scanned light sheet microscopy with confocal slit detection [J].

Baumgart, Eugen ;

Kubitscheck, Ulrich .

OPTICS EXPRESS, 2012, 20 (19) :21805-21814

[2] csiLSFM combines light- sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm [J].

Chang, Bo-Jui ;

Meza, Victor Didier Perez ;

Stelzer, Ernst H. K. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (19) :4869-4874

[3] Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution [J].

Chen, Bi-Chang ;

Legant, Wesley R. ;

Wang, Kai ;

Shao, Lin ;

Milkie, Daniel E. ;

Davidson, Michael W. ;

Janetopoulos, Chris ;

Wu, Xufeng S. ;

Hammer, John A., III ;

Liu, Zhe ;

English, Brian P. ;

Mimori-Kiyosue, Yuko ;

Romero, Daniel P. ;

Ritter, Alex T. ;

Lippincott-Schwartz, Jennifer ;

Fritz-Laylin, Lillian ;

Mullins, R. Dyche ;

Mitchell, Diana M. ;

Bembenek, Joshua N. ;

Reymann, Anne-Cecile ;

Boehme, Ralph ;

Grill, Stephan W. ;

Wang, Jennifer T. ;

Seydoux, Geraldine ;

Tulu, U. Serdar ;

Kiehart, Daniel P. ;

Betzig, Eric .

SCIENCE, 2014, 346 (6208) :439-+

[4]

Cranfill PJ, 2016, NAT METHODS, V13, P557, DOI [10.1038/NMETH.3891, 10.1038/nmeth.3891]

[5] SCANNING LASER MICROSCOPE [J].

DAVIDOVITS, P ;

EGGER, MD .

NATURE, 1969, 223 (5208) :831-+

[6] Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy [J].

Dean, Kevin M. ;

Roudot, Philippe ;

Welf, Erik S. ;

Danuser, Gaudenz ;

Fiolka, Reto .

BIOPHYSICAL JOURNAL, 2015, 108 (12) :2807-2815

[7] Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy [J].

Fahrbach, Florian O. ;

Gurchenkov, Vasily ;

Alessandri, Kevin ;

Nassoy, Pierre ;

Rohrbach, Alexander .

OPTICS EXPRESS, 2013, 21 (09) :11425-11440

[8] Virtual slit scanning microscopy [J].

Fiolka, Reto ;

Stemmer, Andreas ;

Belyaev, Yury .

HISTOCHEMISTRY AND CELL BIOLOGY, 2007, 128 (06) :499-505

[9] Extend the field of view of selective plan illumination microscopy by tiling the excitation light sheet [J].

Gao, Liang .

OPTICS EXPRESS, 2015, 23 (05) :6102-6111

[10] Noninvasive Imaging beyond the Diffraction Limit of 3D Dynamics in Thickly Fluorescent Specimens [J].

Gao, Liang ;

Shao, Lin ;

Higgins, Christopher D. ;

Poulton, John S. ;

Peifer, Mark ;

Davidson, Michael W. ;

Wu, Xufeng ;

Goldstein, Bob ;

Betzig, Eric .

CELL, 2012, 151 (06) :1370-1385

← 1 2 3 →