Universal light-sheet generation with field synthesis

被引：60
作者：
Chang, Bo-Jui [1 ]
Kittisopikul, Mark [2 ,3 ]
Dean, Kevin M. [1 ]
Roudot, Philippe [1 ,4 ]
Welf, Erik S. [1 ,4 ]
Fiolka, Reto [1 ,4 ]
机构：
[1] UT Southwestern Med Ctr, Dept Cell Biol, Dallas, TX 75390 USA
[2] UT Southwestern Med Ctr, Dept Biophys, Dallas, TX USA
[3] Northwestern Univ, Feinberg Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA
[4] UT Southwestern Med Ctr, Lyda Hill Dept Bioinformat, Dallas, TX 75390 USA
来源：
NATURE METHODS | 2019年 / 16卷 / 03期
基金：
美国国家卫生研究院;
关键词：
FLUORESCENCE MICROSCOPY; ILLUMINATION MICROSCOPY; EXCITATION; CELLS; VIEW;
D O I：
10.1038/s41592-019-0327-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We introduce field synthesis, a theorem and method that can be used to synthesize any scanned or dithered light sheet, including those used in lattice light-sheet microscopy (LLSM), from an incoherent superposition of one-dimensional intensity distributions. Compared to LLSM, this user-friendly and modular approach offers a simplified optical design, higher light throughput and simultaneous multicolor illumination. Further, field synthesis achieves lower rates of photobleaching than light sheets generated by lateral beam scanning.
引用
收藏
页码：235 / +
页数：6
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