Protective role of withaferin-A on 7,12-dimethylbenz(a)anthracene-induced genotoxicity in bone marrow of Syrian golden hamsters

被引：19

作者：

Panjamurthy, K. ^{[1
]}

Manoharan, S. ^{[1
]}

Menon, Venugopal P. ^{[1
]}

Nirmal, Madhavan R. ^{[2
]}

Senthil, N. ^{[1
]}

机构：

[1] Annamalai Univ, Dept Biochem & Biotechnol, Fac Sci, Raja Muthaiah Dent Coll & Hosp, Annamalainagar 608002, Tamil Nadu, India

[2] Annamalai Univ, Dept Oral & Maxillofacial Pathol, Raja Muthaiah Dent Coll & Hosp, Annamalainagar 608002, Tamil Nadu, India

来源：

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY | 2008年 / 22卷 / 04期

关键词：

DMBA; withaferin-A; oxidative stress; genotoxicity; chromosomal aberrations;

D O I：

10.1002/jbt.20235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study has investigated the antigenotoxic effect of withaferin-A, a steroidal lactone obtained from the roots and leaves of Withania somnifera, in 7,12-dimethylbenz(a)anthracene (DMBA)induced genotoxicity. Measurement of the frequency of micronucleated polychromatic erythrocytes (MnPCEs) and chromosomal aberrations is used as cytogenetic endpoints. A single intraperitoneal injection of DMBA (30 mg/kg b.w.) to golden Syrian hamsters resulted in marked elevation in the frequency of MnPCEs and aberrations in the chromosomal structure. Hamsters pretreated with withaferin-A intraperitonealy 2 h before the injection of DMBA, significantly reduced the frequency of MnPCEs and chromosomal aberrations such as chromosomal break, gap, minute, and fragment. Our results thus demonstrated the antigenotoxic effect of withaferin-A in DMBA-induced genotoxicity in the bone marrow of golden Syrian hamsters. (C) 2008 Wiley, Periodicals, Inc.

引用

页码：251 / 258

页数：8