Advances in direct methods for protein crystallography

被引：249

作者：

Usón, I ^{[1
]}

Sheldrick, GM ^{[1
]}

机构：

[1] Univ Gottingen, Lehrstuhl Strukturchem, D-37077 Gottingen, Germany

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1999年 / 9卷 / 05期

关键词：

D O I：

10.1016/S0959-440X(99)00020-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in ab initio direct methods have enabled the solution of crystal structures of small proteins from native X-ray data alone, that is, without the use of fragments of known structure or the need to prepare heavy-atom or selenomethionine derivatives, provided that the data are available to atomic resolution. These methods are also proving to be useful for locating the selenium atoms or other anomalous scatterers in the multiple wavelength anomalous diffraction phasing of larger proteins at lower resolution.

引用

页码：643 / 648

页数：6

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