A review on methodologies for extraction, identification and quantification of allergenic proteins in prawns

被引：18
作者：
Faisal, M. [1 ,2 ]
Vasiljevic, T. [1 ,2 ]
Donkor, O. N. [1 ,2 ]
机构：
[1] Victoria Univ, Adv Food Syst Res Unit, Inst Sustainable Ind & Liveable Cities, Werribee Campus,POB 14428, Melbourne, Vic 8001, Australia
[2] Victoria Univ, Coll Hlth & Biomed, Werribee Campus,POB 14428, Melbourne, Vic 8001, Australia
来源：
FOOD RESEARCH INTERNATIONAL | 2019年 / 121卷
关键词：
Prawn allergy; Tropomyosin; Aptamer; Extraction; Quantification; BLACK TIGER PRAWN; MAJOR SHRIMP ALLERGEN; METAPENAEUS-ENSIS TROPOMYOSIN; RESPIRATORY HEALTH SURVEY; CALCIUM-BINDING PROTEIN; PENAEUS-MONODON; OCCUPATIONAL ASTHMA; CROSS-REACTIVITY; SEAFOOD ALLERGY; FOOD ALLERGY;
D O I：
10.1016/j.foodres.2019.03.040
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Prawn allergy is one of the most common food-borne allergies and current prevention is by avoidance. This review paper summarised different methodologies for the extraction, identification and quantification of prawn protein allergens, reported in various research studies. Following extraction, allergenic components have been analysed using well-established methodologies, such as SDS-PAGE, Immunoblotting, ELISA, CD Spectroscopy, HPLC, DBPCFC, SPT etc. Moreover, the preference towards Aptamer-based technique for allergenicity analysis has also been highlighted in this review paper. The summary of these methodologies will provide a reference platform for present and future research directions.
引用
收藏
页码：307 / 318
页数：12
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