Simultaneous angular multiplexing optical projection tomography at shifted focal planes

被引:17
作者
Chen, Lingling [1 ]
Andrews, Natalie [2 ]
Kumar, Sunil [1 ]
Frankel, Paul [3 ]
McGinty, James [1 ]
French, Paul M. W. [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Photon Grp, London SW7 2AZ, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, Inst Chem Biol, London SW7 2AZ, England
[3] UCL, Ctr Cardiovasc Biol & Med, Dept Med, London WC1E 6JJ, England
基金
英国工程与自然科学研究理事会;
关键词
RESOLUTION IMPROVEMENT; MICROSCOPY;
D O I
10.1364/OL.38.000851
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We describe an angular multiplexing technique for optical projection tomography that improves resolution, signal-to-noise ratio, and imaging speed by ameliorating the trade-off between spatial resolution and depth of field and improving the light collection efficiency. Here we demonstrate that imaging at two orthogonal angular projections simultaneously, focused on shifted planes in the sample, improves the average spatial resolution by similar to 20% and the light collection efficiency by a factor of similar to 4, thereby enabling increased acquisition speed and reduced light dose. (C) 2013 Optical Society of America
引用
收藏
页码:851 / 853
页数:3
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