MreB filaments align along greatest principal membrane curvature to orient cell wall synthesis

被引：57

作者：

Hussain, Saman ^{[1
]}

Wivagg, Carl N. ^{[1
]}

Szwedziak, Piotr ^{[2
]}

Wong, Felix ^{[3
]}

Schaefer, Kaitlin ^{[4
]}

Izore, Thierry ^{[2
]}

Renner, Lars D. ^{[5
]}

Holmes, Matthew J. ^{[1
]}

Sun, Yingjie ^{[1
]}

Bisson-Filho, Alexandre W. ^{[1
]}

Walker, Suzanne ^{[6
]}

Amir, Ariel ^{[3
]}

Lowe, Jan ^{[2
]}

Garner, Ethan C. ^{[1
]}

机构：

[1] Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA

[2] MRC, Lab Mol Biol, Cambridge, England

[3] Harvard John A Paulson Sch Engn & Appl Sci, Cambridge, MA USA

[4] Harvard Univ, Dept Microbiol & Immunol, Cambridge, MA 02138 USA

[5] Leibniz Inst Polymer Res Dresden, Dresden, Germany

[6] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

ELIFE | 2018年 / 7卷

基金：

英国惠康基金; 英国医学研究理事会; 美国国家科学基金会; 美国国家卫生研究院;

关键词：

TEICHOIC-ACID POLYMERS; ESCHERICHIA-COLI; WIDESPREAD FAMILY; BACILLUS-SUBTILIS; ACTIN; SHAPE; PEPTIDOGLYCAN; BINDING; RODZ; MORPHOGENESIS;

D O I：

10.7554/eLife.32471

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MreB is essential for rod shape in many bacteria. Membrane-associated MreB filaments move around the rod circumference, helping to insert cell wall in the radial direction to reinforce rod shape. To understand how oriented MreB motion arises, we altered the shape of Bacillus subtilis. MreB motion is isotropic in round cells, and orientation is restored when rod shape is externally imposed. Stationary filaments orient within protoplasts, and purified MreB tubulates liposomes in vitro, orienting within tubes. Together, this demonstrates MreB orients along the greatest principal membrane curvature, a conclusion supported with biophysical modeling. We observed that spherical cells regenerate into rods in a local, self-reinforcing manner: rapidly propagating rods emerge from small bulges, exhibiting oriented MreB motion. We propose that the coupling of MreB filament alignment to shape-reinforcing peptidoglycan synthesis creates a locally-acting, self-organizing mechanism allowing the rapid establishment and stable maintenance of emergent rod shape.

引用

页数：45

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