Distribution of thrombospondins and their neuronal receptor α2β1 in the rat retina

被引:20
|
作者
Huang, Jufang [1 ]
Zhou, Lihong [1 ]
Wang, Hui [1 ]
Luo, Jia [1 ]
Zeng, Leping [1 ]
Xiong, Kun [1 ]
Chen, Dan [1 ]
机构
[1] Cent S Univ, Sch Basic Med Sci, Dept Anat & Neurobiol, Changsha 410013, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
thrombospondin; 1; 2; alpha; delta; retina; synaptic plasticity; HIGH INTRAOCULAR-PRESSURE; CNS SYNAPTOGENESIS; GANGLION-CELLS; EXPRESSION; SUBUNIT; PROTEIN; ASTROCYTES; PLASTICITY; GLAUCOMA; INJURY;
D O I
10.1016/j.exer.2013.03.012
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The role of the extracellular matrix protein thrombospondins (TSPs) in promoting synaptogenesis is gaining more and more attention. The binding of TSP1 and TSP2 to their neuronal receptor alpha 2 delta 1 stimulates excitatory synaptogenesis in the development and injury of the central nervous system; however, the specific cellular localization and expression of TSP1/2 and alpha 2 delta 1 in healthy and damaged retinas is unknown. This, to a certain extent, has restricted the progress of research on the molecular mechanisms triggering synaptic plasticity after retinal injury. Here, the cellular localization and expression of TSP1/2 and their receptor alpha 2 delta 1 was studied in healthy and damaged adult retina induced by elevated intraocular pressure (IOP) using double immunofluorescence labeling and confocal scanning microscopy. We showed the apparent differential distribution of TSP1 and TSP2 in the adult rat retina. TSP1 was confined to the ganglion cell layer and inner nuclear layer, in which it was preferentially expressed by ganglion cells, bipolar cells and horizontal cells but rarely expressed by glial cells. TSP2 staining was diffusely distributed in GFAP- and GS-irnmunopositive glial cells and processes in the inner retina. In rat retinas, alpha 2 delta 1 staining was present in ganglion cells, bipolar cells, partial horizontal cells and amacrine cells and the presynaptic terminals. Muller cells and a minority of astrocytes also expressed alpha 2 delta 1. On the seventh day of elevated IOP, TSP2 immunoreactivity was greatly increased, and immunopositive processes extended throughout the retinal layer and co-localized with GFAP- and GS-positive glial cells. TSP1 distribution in the retina, however, did not change distinctly. alpha 2 delta 1-immunopositive processes were also increased on the seventh day after elevated IOP. Our study suggested that in the adult rat retina, TSP2, but not TSP1, secreted by glial cells may be involved in the synaptic plastic process after retinal injury through binding to its neuronal receptor alpha 2 delta 1. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:36 / 49
页数:14
相关论文
共 50 条
  • [41] NEURONAL DAMAGE IN THE RAT RETINA AFTER CHRONIC STRESS
    OSTEEN, WK
    BRODISH, A
    BRAIN RESEARCH, 1985, 344 (02) : 231 - 239
  • [42] Distribution of melatonin MT1 receptor immunoreactivity in human retina
    Savaskan, E
    Wirz-Justice, A
    Olivieri, G
    Pache, M
    Kräuchi, K
    Brydon, L
    Jockers, R
    Müller-Spahn, F
    Meyer, P
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2002, 50 (04) : 519 - 525
  • [43] Cellular localization and rhythmic expression of melatonin receptor 1 in the rat retina
    Weng, Shi-Jun
    Sheng, Wen-Long
    Yang, Xiong-Li
    Zhong, Yong-Mei
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2014, 55 (13)
  • [44] Differential distribution of dystrophins in rat retina
    Claudepierre, T
    Rodius, F
    Frasson, M
    Fontaine, V
    Picaud, S
    Dreyfus, H
    Mornet, D
    Rendon, A
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1999, 40 (07) : 1520 - 1529
  • [45] Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response
    Ju-Fang Huang
    Lei Shang
    Meng-Qi Zhang
    Hui Wang
    Dan Chen
    Jian-Bin Tong
    He Huang
    Xiao-Xin Yan
    Le-Ping Zeng
    Kun Xiong
    BMC Neuroscience, 14
  • [46] Capsaicin reduces neuronal cell death induced by NMDA via stimulation of opioid receptor in the rat retina
    Sakamoto, Kenji
    Kuroki, Taiyo
    Sekiya, Haruna
    Watanabe, Akihiro
    Mori, Asami
    Nakahara, Tsutomu
    Ishii, Kunio
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2012, 118 : 116P - 116P
  • [47] Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response
    Huang, Ju-Fang
    Shang, Lei
    Zhang, Meng-Qi
    Wang, Hui
    Chen, Dan
    Tong, Jian-Bin
    Huang, He
    Yan, Xiao-Xin
    Zeng, Le-Ping
    Xiong, Kun
    BMC NEUROSCIENCE, 2013, 14
  • [48] Selective synaptic distribution of kainate receptor subunits in the two plexiform layers of the rat retina
    Brandstatter, JH
    Koulen, P
    Wassle, H
    JOURNAL OF NEUROSCIENCE, 1997, 17 (23): : 9298 - 9307
  • [49] The distribution and developmental regulation of NMDA receptor subunit proteins in the outer and inner retina of the rat
    Gründer, T
    Kohler, K
    Kaletta, A
    Guenther, B
    JOURNAL OF NEUROBIOLOGY, 2000, 44 (03): : 333 - 342
  • [50] DISTRIBUTION AND FUNCTION OF CARDIAC ANGIOTENSIN AT(1)-RECEPTOR AND AT(2)-RECEPTOR SUBTYPES IN HYPERTROPHIED RAT HEARTS
    LOPEZ, JJ
    LORELL, BH
    INGELFINGER, JR
    WEINBERG, EO
    SCHUNKERT, H
    DIAMANT, D
    TANG, SS
    AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 267 (02): : H844 - H852