Distribution of thrombospondins and their neuronal receptor α2β1 in the rat retina

被引:20
|
作者
Huang, Jufang [1 ]
Zhou, Lihong [1 ]
Wang, Hui [1 ]
Luo, Jia [1 ]
Zeng, Leping [1 ]
Xiong, Kun [1 ]
Chen, Dan [1 ]
机构
[1] Cent S Univ, Sch Basic Med Sci, Dept Anat & Neurobiol, Changsha 410013, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
thrombospondin; 1; 2; alpha; delta; retina; synaptic plasticity; HIGH INTRAOCULAR-PRESSURE; CNS SYNAPTOGENESIS; GANGLION-CELLS; EXPRESSION; SUBUNIT; PROTEIN; ASTROCYTES; PLASTICITY; GLAUCOMA; INJURY;
D O I
10.1016/j.exer.2013.03.012
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
The role of the extracellular matrix protein thrombospondins (TSPs) in promoting synaptogenesis is gaining more and more attention. The binding of TSP1 and TSP2 to their neuronal receptor alpha 2 delta 1 stimulates excitatory synaptogenesis in the development and injury of the central nervous system; however, the specific cellular localization and expression of TSP1/2 and alpha 2 delta 1 in healthy and damaged retinas is unknown. This, to a certain extent, has restricted the progress of research on the molecular mechanisms triggering synaptic plasticity after retinal injury. Here, the cellular localization and expression of TSP1/2 and their receptor alpha 2 delta 1 was studied in healthy and damaged adult retina induced by elevated intraocular pressure (IOP) using double immunofluorescence labeling and confocal scanning microscopy. We showed the apparent differential distribution of TSP1 and TSP2 in the adult rat retina. TSP1 was confined to the ganglion cell layer and inner nuclear layer, in which it was preferentially expressed by ganglion cells, bipolar cells and horizontal cells but rarely expressed by glial cells. TSP2 staining was diffusely distributed in GFAP- and GS-irnmunopositive glial cells and processes in the inner retina. In rat retinas, alpha 2 delta 1 staining was present in ganglion cells, bipolar cells, partial horizontal cells and amacrine cells and the presynaptic terminals. Muller cells and a minority of astrocytes also expressed alpha 2 delta 1. On the seventh day of elevated IOP, TSP2 immunoreactivity was greatly increased, and immunopositive processes extended throughout the retinal layer and co-localized with GFAP- and GS-positive glial cells. TSP1 distribution in the retina, however, did not change distinctly. alpha 2 delta 1-immunopositive processes were also increased on the seventh day after elevated IOP. Our study suggested that in the adult rat retina, TSP2, but not TSP1, secreted by glial cells may be involved in the synaptic plastic process after retinal injury through binding to its neuronal receptor alpha 2 delta 1. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:36 / 49
页数:14
相关论文
共 50 条
  • [1] DISTRIBUTION OF GLUTAMATE RECEPTOR SUBTYPES IN RAT RETINA
    PENG, YW
    BLACKSTONE, CD
    HUGANIR, RL
    YAU, KW
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1992, 33 (04) : 1174 - 1174
  • [2] Distribution of CB2 Cannabinoid Receptor in Adult Rat Retina
    Lopez, Ester M.
    Tagliaferro, Patricia
    Onaivi, Emmanuel S.
    Lopez-Costa, Juan J.
    SYNAPSE, 2011, 65 (05) : 388 - 392
  • [3] The Role of Astrocyte-Secreted Thrombospondins and Their Neuronal Receptor a2δ-1 in Goal-Directed Actions
    Eroglu, Cagla
    NEUROPSYCHOPHARMACOLOGY, 2023, 48 : 36 - 36
  • [4] The neuronal distribution of cannabinoid receptor type 1 in the trigeminal ganglion of the rat
    Price, TJ
    Helesic, G
    Parghi, D
    Hargreaves, KM
    Flores, CM
    NEUROSCIENCE, 2003, 120 (01) : 155 - 162
  • [5] Distribution of 5-hydroxytriptamine2C receptor mRNA in rat retina
    Pérez-León, JA
    Sarabia, G
    Miledi, R
    García-Alcocer, G
    MOLECULAR BRAIN RESEARCH, 2004, 125 (1-2): : 140 - 142
  • [6] Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina
    Warfvinge, Karin
    Edvinsson, Lars
    Radziwon-Balicka, Aneta
    Blixt, Frank
    CEPHALALGIA, 2017, 37 : 296 - 296
  • [7] Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina
    Blixt, Frank W.
    Radziwon-Balicka, Aneta
    Edvinsson, Lars
    Warfvinge, Karin
    EXPERIMENTAL EYE RESEARCH, 2017, 161 : 124 - 131
  • [8] Expression, distribution and function of kinin B1 receptor in the rat diabetic retina
    Hachana, Soumaya
    Bhat, Menakshi
    Senecal, Jacques
    Huppe-Gourgues, Frederic
    Couture, Rejean
    Vaucher, Elvire
    BRITISH JOURNAL OF PHARMACOLOGY, 2018, 175 (06) : 968 - 983
  • [9] Neurokinin 1 receptor expression in the rat retina
    Casini, G
    Rickman, DW
    Sternini, C
    Brecha, NC
    JOURNAL OF COMPARATIVE NEUROLOGY, 1997, 389 (03) : 496 - 507
  • [10] Neuronal and macrophagic nitric oxide synthase isoforms distribution in normal rat retina
    LopezCosta, JJ
    Goldstein, J
    Saavedra, JP
    NEUROSCIENCE LETTERS, 1997, 232 (03) : 155 - 158