To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus

Acridine orange (AO) and ethidium bromide (EB) are two fluorescent dyes for cell nuclei staining. The nucleus images by these two staining can make it easy to differentiate white blood cell (WBC) from red blood cell (RBC). We applied this principle to the white blood cell count by hemocytometer and this can eliminate the errors caused by misjudging of WBC. At the same time, microscopic imaging can improve the accuracy of the volume measurement. The extended relative uncertainty of two measurements have been analyzed and compared.