To Reduce the Hemocytometer Measurement Uncertainty of White Blood Cell Count by the Double Staining of Cell Nucleus

被引：0
|
作者：
Liu, Ying-Ying [1 ]
Wang, Jing [1 ]
机构：
[1] Natl Inst Metrol, Beijing 100029, Peoples R China
来源：
PROCEEDINGS OF THE 2016 INTERNATIONAL CONFERENCE ON BIOLOGICAL SCIENCES AND TECHNOLOGY | 2016年
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Acridine orange (AO) and ethidium bromide (EB) are two fluorescent dyes for cell nuclei staining. The nucleus images by these two staining can make it easy to differentiate white blood cell (WBC) from red blood cell (RBC). We applied this principle to the white blood cell count by hemocytometer and this can eliminate the errors caused by misjudging of WBC. At the same time, microscopic imaging can improve the accuracy of the volume measurement. The extended relative uncertainty of two measurements have been analyzed and compared.
引用
收藏
页码：14 / 17
页数：4
相关论文
共 50 条
  • [21] VALIDATION OF USE OF THE NAGEOTTE HEMOCYTOMETER TO COUNT LOW-LEVELS OF WHITE CELLS IN WHITE CELL-REDUCED PLATELET COMPONENTS
    MOROFF, G
    EICH, J
    DABAY, M
    TRANSFUSION, 1994, 34 (01) : 35 - 38
  • [22] Dried Blood Spot Glucocerebrosidase Measurement Is Reliable in Controls When Adjusting for White Blood Cell Count
    Ortega, Roberto A.
    Bodamer, Olaf A.
    Mergelsberg, Holly
    Wang, Cuiling
    Glickman, Amanda
    Doan, Nancy
    Raymond, Deborah
    Ratliff, Jeffrey B.
    Squires, Jonathan
    Perera, Imali
    Vaigast, Anna
    Johannes, Brooke
    Bressman, Susan B.
    Saunders-Pullman, Rachel
    ANNALS OF NEUROLOGY, 2016, 80 : S116 - S116
  • [23] White blood cell count and the risk of colon cancer
    Lee, Yong-Jae
    Lee, Hye-Ree
    Nam, Chung-Mo
    Hwang, Ue-Kyoung
    Jee, Sun-Ha
    YONSEI MEDICAL JOURNAL, 2006, 47 (05) : 646 - 656
  • [24] WHITE BLOOD CELL COUNT IN PATIENTS WITH EPILEPTIC SEIZURES
    Papaliagkas, V
    Kazis, D.
    Mavroudis, I
    Daglis, I
    Kolios, S.
    Papagiannopoulos, S.
    Bostantjopoulou, S.
    EPILEPSIA, 2014, 55 : 203 - 203
  • [25] DAILY RHYTHM OF WHITE BLOOD CELL COUNT IN PREGNANCY
    MALEK, J
    ACTA MEDICA ACADEMIAE SCIENTIARUM HUNGARICAE, 1961, 17 (02) : 193 - &
  • [26] Epistaxis, Ecchymoses, and an Abnormal White Blood Cell Count
    Sellers, Meredith H.
    Dinner, Shira N.
    JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2017, 318 (04): : 383 - 384
  • [27] The variations of white blood cell count in Lipizzan horses
    Cebulj-Kadunc, N
    Kosec, M
    Cestnik, V
    JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 2003, 50 (05): : 251 - 253
  • [28] White blood cell count as an indicator of formaldehyde exposure
    Kuo, HW
    Jian, GJ
    Chen, CL
    Liu, CS
    Lai, JS
    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1997, 59 (02) : 261 - 267
  • [29] White blood cell count in the course of typhoid fever
    Lefebvre, N.
    Gning, S.-B.
    Ndiaye, A.
    Ba-Fall, K.
    Debonne, J.-M.
    MEDECINE ET MALADIES INFECTIEUSES, 2007, 37 (03): : 178 - 179
  • [30] ChlormethineReduced white blood cell count: case report
    Reactions Weekly, 2014, 1506 (1) : 11 - 11
12345