Specificity and Heterogeneity of Terahertz Radiation Effect on Gene Expression in Mouse Mesenchymal Stem Cells

被引:84
作者
Alexandrov, Boian S. [1 ,2 ]
Phipps, M. Lisa [3 ]
Alexandrov, Ludmil B. [4 ]
Booshehri, Layla G. [5 ]
Erat, Anna [2 ,6 ]
Zabolotny, Janice [2 ]
Mielke, Charles H. [5 ]
Chen, Hou-Tong [3 ]
Rodriguez, George [3 ]
Rasmussen, Kim O. [1 ]
Martinez, Jennifer S. [3 ]
Bishop, Alan R. [1 ]
Usheva, Anny [2 ]
机构
[1] Los Alamos Natl Lab, Div Theoret, Los Alamos, NM 87545 USA
[2] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USA
[3] Los Alamos Natl Lab, Ctr Integrated Nanotechnol, Los Alamos, NM 87545 USA
[4] Wellcome Trust Sanger Inst, Canc Genome Project, Cambridge CB10 1SA, England
[5] Los Alamos Natl Lab, Mat Phys & Applicat Div NHMFL, Los Alamos, NM 87545 USA
[6] Univ Zurich Hosp, Res Unit, Div Internal Med, Zurich, Switzerland
来源
SCIENTIFIC REPORTS | 2013年 / 3卷
关键词
DNA BREATHING DYNAMICS; TRANSCRIPTION FACTOR; SPINDLE DISTURBANCES; DIFFERENTIATION; GENERATION; BIOLOGY; DIRECTS; MODEL;
D O I
10.1038/srep01184
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report that terahertz (THz) irradiation of mouse mesenchymal stem cells (mMSCs) with a single-frequency (SF) 2.52 THz laser or pulsed broadband (centered at 10 THz) source results in irradiation specific heterogenic changes in gene expression. The THz effect depends on irradiation parameters such as the duration and type of THz source, and on the degree of stem cell differentiation. Our microarray survey and RT-PCR experiments demonstrate that prolonged broadband THz irradiation drives mMSCs toward differentiation, while 2-hour irradiation (regardless of THz sources) affects genes transcriptionally active in pluripotent stem cells. The strictly controlled experimental environment indicates minimal temperature changes and the absence of any discernable response to heat shock and cellular stress genes imply a non-thermal response. Computer simulations of the core promoters of two pluripotency markers reveal association between gene upregulation and propensity for DNA breathing. We propose that THz radiation has potential for non-contact control of cellular gene expression.
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页数:8
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