Construction of Asymmetric Cell-Sized Lipid Vesicles from Lipid-Coated Water-in-Oil Microdroplets

被引：87

作者：

Hamada, Tsutomu ^{[1
]}

Miura, Yoko ^{[1
]}

Komatsu, Yuuki ^{[1
]}

Kishimoto, Yuko ^{[1
]}

Vestergaard, Mun'delanji ^{[1
]}

Takagi, Masahiro ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2008年 / 112卷 / 47期

基金：

日本学术振兴会;

关键词：

D O I：

10.1021/jp807784j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.

引用

页码：14678 / 14681

页数：4

共 29 条

[1] CONSTRUCTION OF ASYMMETRIC CELL-SIZED LIPID VESICLES FROM LIPID-COATED WATER-IN-OIL MICRODROPLETS
Takagi, Masahiro
Hamada, Tsutomu
Vestergaard, Mun'delanji
IFPT'6: PROGRESS ON POST-GENOME TECHNOLOGIES, PROCEEDINGS, 2009, : 31 - 35
[2] Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes
Kamiya K.
Kawano R.
Osaki T.
Akiyoshi K.
Takeuchi S.
Nature Chemistry, 2016, 8 (9) : 881 - 889
[3] Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes
Kamiya, Koki
Kawano, Ryuji
Osaki, Toshihisa
Akiyoshi, Kazunari
Takeuchi, Shoji
NATURE CHEMISTRY, 2016, 8 (09) : 881 - 889
[4] Gene expression within cell-sized lipid vesicles
Nomura, S
Tsumoto, K
Hamada, T
Akiyoshi, K
Nakatani, Y
Yoshikawa, K
CHEMBIOCHEM, 2003, 4 (11) : 1172 - 1175
[5] Effects of isovaleraldehyde on cell-sized lipid bilayer vesicles
Yoda, Tsuyoshi
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BIOPHYSICAL CHEMISTRY, 2021, 279
[6] Dynamical formation of lipid bilayer vesicles from lipid-coated droplets across a planar monolayer at an oil/water interface
Ito, Hiroaki
Yamanaka, Toru
Kato, Shou
Hamada, Tsutomu
Takagi, Masahiro
Ichikawa, Masatoshi
Yoshikawa, Kenichi
SOFT MATTER, 2013, 9 (40) : 9539 - 9547
[7] Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles
Fei Tao
Peng Yang
Scientific Reports, 5
[8] Ca-Mediated Electroformation of Cell-Sized Lipid Vesicles
Tao, Fei
Yang, Peng
SCIENTIFIC REPORTS, 2015, 5
[9] Cell penetrating peptides facilitate protein transport into the cell-sized asymmetric lipid vesicles at arbitrary timing
Miwa, Akari
Kamiya, Koki
BIOPHYSICAL JOURNAL, 2023, 122 (03) : 368A - 368A
[10] Confined Motion: Motility of Active Microparticles in Cell-Sized Lipid Vesicles
Song, Shidong
Llopis-Lorente, Antoni
Mason, Alexander F.
Abdelmohsen, Loai K. E. A.
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JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2022, 144 (30) : 13831 - 13838

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