Construction of Asymmetric Cell-Sized Lipid Vesicles from Lipid-Coated Water-in-Oil Microdroplets

被引：87

作者：

Hamada, Tsutomu ^{[1
]}

Miura, Yoko ^{[1
]}

Komatsu, Yuuki ^{[1
]}

Kishimoto, Yuko ^{[1
]}

Vestergaard, Mun'delanji ^{[1
]}

Takagi, Masahiro ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Nomi, Ishikawa 9231292, Japan

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2008年 / 112卷 / 47期

基金：

日本学术振兴会;

关键词：

D O I：

10.1021/jp807784j

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We present a simple, rapid, and robust method for preparing asymmetric cell-sized lipid bilayer vesicles using water-in-oil (W/O) microdroplets transferred through an oil-water interface. The efficiency for producing cell-sized model membranes is elucidated in relation to the vesicular size and the weight of contained water-soluble molecules. We demonstrate the biological asymmetric nature and the formation of lipid raft microdomain structures using fluorescence microscopy.

引用

页码：14678 / 14681

页数：4

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