Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation

被引:5
作者
Kishino, K
Muroi, K [1 ]
Kawano, C
Obata, T
Sugano, N
Nakagi, Y
Nagashima, T
Watari, K
Iwamoto, S
Ozawa, K
机构
[1] Jichi Med Sch, Div Cell Transplantat & Transfus, Minami Kawachi, Tochigi 3290498, Japan
[2] Jichi Med Sch, Dept Hematol, Minami Kawachi, Tochigi 3290498, Japan
[3] Jichi Med Sch, Dept Legal Med & Human Genet, Minami Kawachi, Tochigi 3290498, Japan
关键词
bone marrow transplantation; engraftment; ABO genotype; erythroid burst-forming units;
D O I
10.1016/S0145-2126(01)00090-X
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Six patients received an allogeneic bone marrow transplant from HLA-identical ABO-mismatched donors. ABO genotype of erythroid burst-forming units (BFU-E) from peripheral blood was analyzed using polymerase chain reaction with sequence specific primers (PCR-SSP). After bone marrow transplantation (BMT), engraftment of donor cells by ABO genotypic analysis of BFU-E was compared with ABO phenotypic analysis of red blood cells (RBCs). During the early stage after BMT, ABO genotype of BFU-E in the recipients converted to that of the donors. In contrast, mixed ABO phenotype of RBCs persisted for about 3 months. In one patient, autologous hemopoietic cell recovery was detected by the ABO genotypic analysis before clinical manifestation. ABO genotypic analysis of BFU-E is relevant for enagraftment after ABO-mismatched BMT. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:13 / 17
页数:5
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