Levels of VEGF-A mRNA in plasma from patients with colorectal carcinoma as possible surrogate marker of angiogenesis

被引：13

作者：

Garcia, Vanesa ^{[1
]}

Garcia, Jose M. ^{[1
]}

Silva, Javier ^{[1
]}

Pena, Cristina ^{[1
]}

Dominguez, Gemma ^{[1
]}

Lorenzo, Yolanda ^{[1
]}

Diaz, Raquel ^{[1
]}

Alonso, Isabel ^{[2
]}

Colas, Antonio ^{[2
]}

Hurtado, Alicia ^{[1
]}

Sanchez, Antonio ^{[1
]}

Bonilla, Felix ^{[1
]}

机构：

[1] Hosp Univ Puerta Hierro, Dept Med Oncol, Madrid 28035, Spain

[2] Hosp Univ Puerta Hierro, Dept Surg, Madrid 28035, Spain

来源：

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | 2008年 / 134卷 / 11期

关键词：

VEGF-A; mRNA; plasma; colorectal cancer;

D O I：

10.1007/s00432-008-0405-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose The regulator of angiogenesis most extensively studied is VEGF. VEGF mRNA in plasma from patients with colorectal cancer was analyzed as a possible surrogate marker of tumor angiogenesis. Methods VEGF mRNA was measured by quantitative PCR in plasma, tumors and circulating tumor cells from colorectal cancer patients. Circulating VEGF protein was analyzed by ELISA. Microvessel density was determined. Results Levels of VEGF mRNA and protein in plasma were higher in patients than in controls. VEGF mRNA was overexpressed in tumors with respect to normal tissues. Levels of VEGF protein were associated with VEGF mRNA in plasma, but no associations with tumor samples were found. A trend to statistical significance was shown between high VEGF mRNA and vascular invasion. MVD was not related to VEGF mRNA in plasma. Conslusions Thus, VEGF mRNA could be a marker similar to VEGF protein in plasma.

引用

页码：1165 / 1171

页数：7

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