Extracellular Matrix Patterning for Cell Alignment by Atmospheric Pressure Plasma Jets

被引:8
作者
Ando, Ayumi [1 ]
Asano, Toshifumi [2 ]
Abu Sayed, Md [3 ]
Tero, Ryugo [4 ]
Kitano, Katsuhisa [1 ]
Urisu, Tsuneo [5 ]
Hamaguchi, Satoshi [1 ]
机构
[1] Osaka Univ, Ctr Atom & Mol Technol, Suita, Osaka 5650871, Japan
[2] Tohoku Univ, Dept Dev Biol & Neurosci, Div Neurosci, Sendai, Miyagi 9808577, Japan
[3] Grad Univ Adv Studies, Dept Struct Mol Sci, Okazaki, Aichi 4448585, Japan
[4] Toyohashi Univ Technol, Elect Inspired Interdisciplinary Res Inst, Toyohashi, Aichi 4418580, Japan
[5] Nagoya Univ, FIRST Res Ctr Innovat Nanobiodevice, Nagoya, Aichi 4648603, Japan
关键词
FIBRONECTIN; ADHESION; CULTURE; MICROPATTERNS; FABRICATION; ADSORPTION; BIOSENSOR; PROTEINS; COATINGS;
D O I
10.1143/JJAP.51.036201
中图分类号
O59 [应用物理学];
学科分类号
摘要
Low-temperature atmospheric-pressure plasma (APP) jets and a metal stencil mask have been used for the patterning of fibronectins deposited on a silicon (Si) wafer. Fibronectins typically constitute the extracellular matrix (ECM) and a micro-patterned ECM may be used for arranging living cells in a desired pattern on the substrate surface. Such a technique can be used for the fabrication of cell chips. In this study, patterning of 100-mu m-wide lines of fibronectin layers has been demonstrated. Desorption of fibronectins from the surface by plasma application has been confirmed by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FT-IR). (C) 2012 The Japan Society of Applied Physics
引用
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页数:7
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