Ethanol Inactivated Mouse Embryonic Fibroblasts Maintain the Self-Renew and Proliferation of Human Embryonic Stem Cells

被引：5
作者：
Huang, Boxian [1 ,2 ]
Ning, Song [2 ]
Zhuang, Lili [3 ]
Jiang, Chunyan [2 ]
Cui, Yugui [2 ]
Fan, Guoping [4 ]
Qin, Lianju [2 ]
Liu, Jiayin [1 ,2 ]
机构：
[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210038, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Affiliated Hosp 1, Ctr Clin Reprod Med, State Key Lab Reprod Med, Nanjing 210029, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Affiliated Hosp 1, Dept Pediat, Nanjing 210029, Jiangsu, Peoples R China
[4] Univ Calif Los Angeles, David Geffen Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA
来源：
PLOS ONE | 2015年 / 10卷 / 06期
基金：
中国国家自然科学基金;
关键词：
SYNTHETIC-POLYMER COATINGS; HEPARAN-SULFATE; CULTURE; GROWTH; DIFFERENTIATION; DERIVATION; IMMOBILIZATION; PLURIPOTENCY; INDUCTION; PROTEINS;
D O I：
10.1371/journal.pone.0130332
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Conventionally, mouse embryonic fibroblasts (MEFs) inactivated by mitomycin C or irradiation were applied to support the self-renew and proliferation of human embryonic stem cells (hESCs). To avoid the disadvangtages of mitomycin C and irradiation, here MEFs were treated by ethanol (ET). Our data showed that 10% ET-inactivated MEFs (eiMEFs) could well maintain the self-renew and proliferation of hESCs. hESCs grown on eiMEFs expressed stem cell markers of NANOG, octamer-binding protein 4 (OCT4), stage-specific embryonic antigen-4 (SSEA4) and tumour related antigen-1-81 (TRA-1-81), meanwhile maintained normal karyotype after long time culture. Also, hESCs cocultured with eiMEFs were able to form embryoid body (EB) in vitro and develop teratoma in vivo. Moreover, eiMEFs could keep their nutrient functions after long time cryopreservation. Our results indicate that the application of eiMEF in hESCs culture is safe, economical and convenient, thus is a better choice.
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页数：14
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