Molecular characterisation of a putative new polerovirus infecting pumpkin (Cucurbita pepo) in Kenya

被引：10

作者：

Kidanemariam, Dawit B. ^{[1
,2
]}

Sukal, Am It C. ^{[1
]}

Abraham, Adane D. ^{[3
]}

Njuguna, Joyce N. ^{[4
]}

Stomeo, Francesca ^{[4
]}

Dale, James L. ^{[1
]}

Harding, Robert M. ^{[1
]}

James, Anthony P. ^{[1
]}

机构：

[1] Queensland Univ Technol, Ctr Trop Crops & Biocommod, Brisbane, Qld 4001, Australia

[2] Ethiopian Inst Agr Res, Natl Agr Biotechnol Res Ctr, Addis Ababa, Ethiopia

[3] Addis Ababa Sci & Technol Univ, Dept Biotechnol, Addis Ababa, Ethiopia

[4] Biosci Eastern & Cent Africa Int Livestock Res In, Nairobi, Kenya

来源：

ARCHIVES OF VIROLOGY | 2019年 / 164卷 / 06期

关键词：

VIRUS;

D O I：

10.1007/s00705-019-04219-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Next-generation sequencing of RNA extracted from a pumpkin plant with mosaic symptoms in Kenya identified the presence of a polerovirus sequence closely related to pepo aphid-borne yellows virus (PABYV). The near-complete polerovirus sequence comprised 5,810 nucleotides and contained seven putative open reading frames (ORFs) with a genome organisation typical of poleroviruses. BLASTp analysis of the translated sequences of ORFs 0, 1 and 2 revealed that their amino acid sequences differed by more than 10% from the corresponding protein sequences of other poleroviruses. These results suggest that this virus is a putative novel member of the genus Polerovirus, which has been provisionally named pumpkin polerovirus (PuPV).

引用

页码：1717 / 1721

页数：5