Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos

被引：35

作者：

Bohorova, N ^{[1
]}

Zhang, W ^{[1
]}

Julstrum, P ^{[1
]}

McLean, S ^{[1
]}

Luna, B ^{[1
]}

Brito, RM ^{[1
]}

Diaz, L ^{[1
]}

Ramos, ME ^{[1
]}

Estanol, P ^{[1
]}

Pacheco, M ^{[1
]}

Salgado, M ^{[1
]}

Hoisington, D ^{[1
]}

机构：

[1] CIMMYT, Mexico City 06600, DF, Mexico

来源：

THEORETICAL AND APPLIED GENETICS | 1999年 / 99卷 / 3-4期

关键词：

tropical maize; transformation; plant regeneration; insect resistance; Bacillus thuringiensis (Bt);

D O I：

10.1007/s001220051255

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

To enhance the level of resistance to insects in tropical maize germplasm we have developed techniques to successfully transform elite tropical maize inbred based on the activity of specific cryI proteins against four major maize pests - corn earworm, fall armyworm, southwestern corn borer and sugarcane borer. Constructs containing cryIAb or cryIAc synthetic genes were used. To generate transgenic plants we have established methods for biolistic bombardment and the selection and regeneration of immature embryos and calli from the elite tropical lines CML72, CML216, CML323, CML327 and hybrids. Transgenic plants resistant to the herbicide Basta(TM) contained the bands for the cry, bar and gus genes as detected by Southern blot analyses. A simple leaf bioassay presented varying levels of resistance to Southwestern corn borer of transgenic tropical maize carrying the cryIAc gene. Analyses of the progenies confirmed the sexual transmission of the introduced genes and their stable expression.

引用

页码：437 / 444

页数：8

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