The interaction between purple membrane and membrane lipid

被引：0
|
作者：
Hu, KS [1 ]
机构：
[1] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2002年 / 47卷 / 07期
基金：
英国惠康基金; 中国国家自然科学基金;
关键词：
purple membrane; bacteriorhodopsin (BR); lipid vesicle;
D O I：
10.1360/02tb9129
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Bacteriorhodopsin in purple membrane was reconstituted into different lipid vesicles. The effect of three different lipids on the structure and function of bacteriorhodopsin in lipid vesicles was studied by the observation on freeze-fracture eletron microscopy, the rotational diffusion of bacteriorhodopsin in lipid vesicles, the measurement of absorption spectrum, and the absorbance change with time. For these prepared samples, the results showed that DMPC was the stable lipid environment of bacteriorhodopsin; egg-pe causeed the loss of retinal chromophore of bacteriorhodopsin and it was not reversible change, cholesterol could stabilize the bacteriorhodopsin in lipid environment, but it caused the aggregation of bacteriorhodopsin.
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页码：562 / 564
页数：3
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