Evaluation of arginine metabolism for the analysis of M1/M2 macrophage activation in human clinical specimens

被引：22

作者：

Geelhaar-Karsch, Anika ^{[1
,2
]}

Schinnerling, Katina ^{[1
,2
]}

Conrad, Kristina ^{[1
]}

Friebel, Julian ^{[1
]}

Allers, Kristina ^{[1
]}

Schneider, Thomas ^{[1
]}

Moos, Verena ^{[1
]}

机构：

[1] Charite, Med Klin 1, D-12203 Berlin, Germany

[2] FU Berlin, Fachbereich Biol, Berlin, Germany

来源：

INFLAMMATION RESEARCH | 2013年 / 62卷 / 09期

关键词：

Macrophage function and differentiation; Nitrite detection; Arginase activity; Whipple's disease; NITRIC-OXIDE SYNTHASE; MURINE MACROPHAGES; ARGINASE ACTIVITY; WHIPPLES-DISEASE; CELLS; POLARIZATION; EXPRESSION;

D O I：

10.1007/s00011-013-0642-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Macrophage heterogeneity reflects their plasticity in response to environmental stimuli. Usually human macrophages are characterized by analysis of surface molecules or cytokine expression while functional assays are established in the mouse system but lacking for various human specimens. To evaluate the value of analysis of arginine metabolism for characterization of human macrophage differentiation, we analyzed nitrite production and arginase activity in plasma, duodenal biopsies, and in vitro differentiated macrophages of patients with classical Whipple's disease. We demonstrate that it is feasible to determine the content of urea in supernatants of stimulated duodenal biopsies, arginase activity in fresh duodenal biopsies and plasma samples, and arginase activity and nitrite production in lysates and supernatants of in vitro differentiated macrophages. However, only selected tests are appropriate to define macrophage polarization in human specimens. Analysis of arginine metabolism is not suitable for the characterization of in vitro differentiated human macrophages. Besides the measurement of nitrite in duodenal biopsy supernatants, the determination of arginase activity in human plasma seems to be a reasonable functional test to detect enhanced M2 macrophage activation and, thus, is of great value for the analysis of macrophage activity with a minimum of material and costs.

引用

页码：865 / 869

页数：5

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