Optimization of Process Parameters for the Production of Cellulases under Solid State Fermentation

被引：0

作者：

Jadon, Nitesh ^{[2
]}

Devi, Nirmala ^{[1
]}

Garg, Shashank ^{[2
]}

Kumar, Anupam ^{[2
]}

Khasa, Yogender Pal ^{[1
]}

Kumar, Manoj ^{[2
]}

机构：

[1] Univ Delhi, Dept Microbiol, New Delhi 11021, India

[2] Lovely Profess Univ, Dept Biotechnol, Phagwara 144402, Punjab, India

来源：

JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2013年 / 7卷 / 01期

关键词：

Cellulases; Solid State Fermentation; Opimization; FPase; CMCase; beta-glucosidase; ENZYMES;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cellulolytic fungal strain was successfully isolated from local region and further subjected to solid state fermentation (SSF). Optimization of production parameters were done at shake flask level to apply at production scale. Low cost approach was determined for commercial viability of such industrial enzyme. Basic as well as applied parameters were optimized for better production. The maximum activity were recorded as 2.9 U/mL, 8.3 U/mL and 110 U/mL for FPase, CMCase and beta-glucosidase respectively at 30 C and pH 6 with wheat bran as substrate with 20% (w/w) inoculums concentration on 5th day of incubation. These parameters can further be used as benchmark for upscaling of the process for commercial production under SSF conditions.

引用

页码：653 / 660

页数：8

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