Microstereolithography-Based Fabrication of Anatomically Shaped Beta-Tricalcium Phosphate Scaffolds for Bone Tissue Engineering

被引:13
作者
Du, Dajiang [1 ,2 ]
Asaoka, Teruo [3 ]
Shinohara, Makoto [2 ]
Kageyama, Tomonori [2 ]
Ushida, Takashi [4 ]
Furukawa, Katsuko Sakai [2 ]
机构
[1] Harbin Med Univ, Sino Russian Inst Hard Tissue Dev & Regenerat, Dept Orthopaed Surg, Harbin 150086, Peoples R China
[2] Univ Tokyo, Dept Bioengn, Bunkyo Ku, Tokyo 1138656, Japan
[3] Tokyo Denki Univ, Dept Mech Engn, Adachi Ku, Tokyo 1018457, Japan
[4] Univ Tokyo, Ctr Dis Biol & Integrat Med, Bunkyo Ku, Tokyo 1138656, Japan
基金
中国国家自然科学基金;
关键词
MICRO-STEREOLITHOGRAPHY; CERAMIC SCAFFOLDS; 3D; DESIGN; CELLS;
D O I
10.1155/2015/859456
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Porous ceramic scaffolds with shapes matching the bone defects may result in more efficient grafting and healing than the ones with simple geometries. Using computer-assisted microstereolithography (MSTL), we have developed a novel gelcasting indirect MSTL technology and successfully fabricated two scaffolds according to CT images of rabbit femur. Negative resin molds with outer 3D dimensions conforming to the femur and an internal structure consisting of stacked meshes with uniform interconnecting struts, 0.5mm in diameter, were fabricated by MSTL. The second mold type was designed for cortical bone formation. A ceramic slurry of beta-tricalcium phosphate (beta-TCP) with room temperature vulcanization (RTV) silicone as binder was cast into the molds. After the RTV silicone was completely cured, the composite was sintered at 1500 degrees C for 5 h. Both gross anatomical shape and the interpenetrating internal network were preserved after sintering. Even cortical structure could be introduced into the customized scaffolds, which resulted in enhanced strength. Biocompatibility was confirmed by vital staining of rabbit bone marrow mesenchymal stromal cells cultured on the customized scaffolds for 5 days. This fabrication method could be useful for constructing bone substitutes specifically designed according to local anatomical defects.
引用
收藏
页数:9
相关论文
共 32 条
[1]   Lost Mold Rapid Infiltration Forming of Mesoscale Ceramics: Part 1, Fabrication [J].
Antolino, Nicholas E. ;
Hayes, Gregory ;
Kirkpatrick, Rebecca ;
Muhlstein, Christopher L. ;
Frecker, Mary I. ;
Mockensturm, Eric M. ;
Adair, James H. .
JOURNAL OF THE AMERICAN CERAMIC SOCIETY, 2009, 92 (01) :S63-S69
[2]   COMPRESSIVE BEHAVIOR OF BONE AS A 2-PHASE POROUS STRUCTURE [J].
CARTER, DR ;
HAYES, WC .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1977, 59 (07) :954-962
[3]  
Cho DW, 2012, METHODS MOL BIOL, V868, P341, DOI 10.1007/978-1-61779-764-4_21
[4]   Gel-cast glass-ceramic tissue scaffolds of controlled architecture produced via stereolithography of moulds [J].
Chopra, K. ;
Mummery, P. M. ;
Derby, B. ;
Gough, J. E. .
BIOFABRICATION, 2012, 4 (04)
[5]   Hydroxyapatite implants with designed internal architecture [J].
Chu, TMG ;
Halloran, JW ;
Hollister, SJ ;
Feinberg, SE .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2001, 12 (06) :471-478
[6]   Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth [J].
Cooke, MN ;
Fisher, JP ;
Dean, D ;
Rimnac, C ;
Mikos, AG .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2003, 64B (02) :65-69
[7]  
Dorozhkin SV, 2002, ANGEW CHEM INT EDIT, V41, P3130, DOI 10.1002/1521-3773(20020902)41:17<3130::AID-ANIE3130>3.0.CO
[8]  
2-1
[9]  
Du D., 2014, J BIOMEDICAL MAT R B, V103, P84
[10]   3D Culture of Osteoblast-Like Cells by Unidirectional or Oscillatory Flow for Bone Tissue Engineering [J].
Du, Dajiang ;
Furukawa, Katsuko S. ;
Ushida, Takashi .
BIOTECHNOLOGY AND BIOENGINEERING, 2009, 102 (06) :1670-1678