Lack of Na+,K+-ATPase expression in intercalated cells may be compensated by Na+-ATPase:: A study on MDCK-C11 cells

被引：7
作者：
Sampaio, M. S. [1 ]
Bezerra, I. P. [1 ]
Pecanha, F. L. [1 ]
Fonseca, P. H. [1 ]
Capella, M. A. M. [1 ]
Lopes, A. G. [1 ]
机构：
[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21941 Rio De Janeiro, Brazil
来源：
CELLULAR AND MOLECULAR LIFE SCIENCES | 2008年 / 65卷 / 19期
关键词：
Na+; K+-ATPase; Na+-ATPase; MDCK-C11; MDCK-C7; intercalated cells;
D O I：
10.1007/s00018-008-8393-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The lack of Na+,K+-ATPase expression in intercalated cells (IC) is an intriguing condition due to its fundamental role in cellular homeostasis. In order to better understand this question we compared the activities of Na+,K+-ATPase and Na+-ATPase in two MDCK cell clones: the C11, with IC characteristics, and the C7, with principal cells (PC) characteristics. The Na+,K+-ATPase activity found in C11 cells is far lower than in C7 cells and the expression of its beta-subunit is similar in both cells. On the other hand, a subset of C11 without alpha-subunit expression has been found. In C11 cells the Na+-ATPase activity is higher than that of the Na+,K+-ATPase, and it is increased by medium alkalinization, suggesting that it could account for the cellular Na+-homeostasis. Although further studies are necessary for a better understanding of these findings, the presence of Na+-ATPase may explain the adequate survival of cells that lack Na+,K+-ATPase.
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收藏
页码：3093 / 3099
页数：7
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