Liquid chromatography tandem mass spectrometry method for the quantification of clonidine with LLOQ of 10 pg/mL in human plasma

被引:8
作者
Nirogi, Ramakrishna [1 ]
Kandikere, Vishwottam [1 ]
Mudigonda, Koteshwara [1 ]
Komarneni, Prashanth [1 ]
机构
[1] Suven Life Sci Ltd, Biopharmaceut Res, Hyderabad 500034, Andhra Pradesh, India
关键词
clonidine; liquid chromatography-tandem mass spectrometry; human plasma; pharmacokinetic study;
D O I
10.1002/bmc.1018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive high-performance liquid chromatography-positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of clonidine in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H](+) ions, m/z 230 to 44 for clonidine and m/z 254 to 44 for the internal standard. The assay exhibited a linear dynamic range of 10-2000 pg/mL for clonidine in human plasma. The lower limit of quantification was 10 pg/mL with a relative standard deviation of less than 6.8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.5 min for each sample made it possible to analyze more than 250 human plasma samples per day. The validated method was successfully used to analyze human plasma samples for application in pharmacokinetic studies. Copyright (C) 2008 John Wiley & Sons, Ltd.
引用
收藏
页码:992 / 1000
页数:9
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