Evaluation of different methods for immobilization of Candida antarctica lipase B (CalB lipase) in polyurethane foam and its application in the production of geranyl propionate

被引：44

作者：

Nicoletti, Gabrieli ^{[1
]}

Cipolatti, Eliane P. ^{[1
]}

Valerio, Alexsandra ^{[1
]}

Carbonera, NataliaThaisa Gamba ^{[1
]}

Soares, Nicole Spillere ^{[1
]}

Theilacker, Eron ^{[1
]}

Ninow, Jorge L. ^{[1
]}

de Oliveira, Debora ^{[1
]}

机构：

[1] Univ Fed Santa Catarina, Chem & Food Engn Dept, BR-88040900 Florianopolis, SC, Brazil

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2015年 / 38卷 / 09期

关键词：

Lipase; Polyurethane foam; Esters; Geranyl propionate; SOLVENT-FREE SYSTEM; ENZYMATIC-ACTIVITY; RUGOSA LIPASE; ORGANIC MEDIA; STABILIZATION; POLYETHYLENEIMINE; NANOPARTICLES; BIOCATALYSTS; STABILITY; SUPPORTS;

D O I：

10.1007/s00449-015-1415-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

With the aim of studying the best method for the interaction of polyurethane (PU) foam and Candida antarctica lipase B, different methods of CalB immobilization were studied: adsorption (PU-ADS), bond (using polyethyleneimine) (PU-PEI), ionic adsorption by PEI with cross-linking with glutaraldehyde (PU-PEI-GA) and entrapment (PU). The characterization of immobilized enzyme derivatives was performed by apparent density and Fourier transform infrared spectroscopy. The free enzyme and enzyme preparations were evaluated at different pH values and temperatures. The highest enzyme activity was obtained using the PU method (5.52 U/g). The methods that stood out to compare the stabilities and kinetic parameters were the PU and PU-ADS. Conversions of 83.5 and 95.9 % for PU and PU-ADS derivatives were obtained, in 24 h reaction, using citronella oil and propionic acid as substrates.

引用

页码：1739 / 1748

页数：10

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