Angiotensin-Converting Enzyme 2 in the Rostral Ventrolateral Medulla Regulates Cholinergic Signaling and Cardiovascular and Sympathetic Responses in Hypertensive Rats

被引：17
作者：
Deng, Yu [1 ,3 ]
Tan, Xing [1 ,2 ]
Li, Miao-Ling [4 ]
Wang, Wei-Zhong [1 ,2 ]
Wang, Yang-Kai [1 ,2 ]
机构：
[1] Second Mil Med Univ, Dept Physiol, Shanghai 200433, Peoples R China
[2] Second Mil Med Univ, Inst Polar Med Res Ctr, Shanghai 200433, Peoples R China
[3] Changhai Hosp, Dept Anesthesiol, Shanghai 200433, Peoples R China
[4] Southwest Med Univ, Inst Cardiovasc Med Res, Luzhou 646000, Peoples R China
来源：
NEUROSCIENCE BULLETIN | 2019年 / 35卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Hypertension; Renin-angiotensin system; Gene transfer; Acetylcholine; Sympathetic nerve activity; ACTIVE GLUTAMATERGIC INPUT; BLOOD-PRESSURE REGULATION; ARTERIAL-PRESSURE; BRAIN; RECEPTOR; MECHANISMS; NEURONS; OVEREXPRESSION; ACETYLCHOLINE; BALANCE;
D O I：
10.1007/s12264-018-0298-3
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The rostral ventrolateral medulla (RVLM) is a key region in cardiovascular regulation. It has been demonstrated that cholinergic synaptic transmission in the RVLM is enhanced in hypertensive rats. Angiotensin-converting enzyme 2 (ACE2) in the brain plays beneficial roles in cardiovascular function in hypertension. The purpose of this study was to determine the effect of ACE2 overexpression in the RVLM on cholinergic synaptic transmission in spontaneously hypertensive rats (SHRs). Four weeks after injecting lentiviral particles containing enhanced green fluorescent protein and ACE2 bilaterally into the RVLM, the blood pressure and heart rate were notably decreased. ACE2 overexpression significantly reduced the concentration of acetylcholine in microdialysis fluid from the RVLM and blunted the decrease in blood pressure evoked by bilateral injection of atropine into the RVLM in SHRs. In conclusion, we suggest that ACE2 overexpression in the RVLM attenuates the enhanced cholinergic synaptic transmission in SHRs.
引用
收藏
页码：67 / 78
页数：12
相关论文
共 62 条
[1]   Chronic exercise modulates RAS components and improves balance between pro- and anti-inflammatory cytokines in the brain of SHR [J].
Agarwal, Deepmala ;
Welsch, Michael A. ;
Keller, Jeffrey N. ;
Francis, Joseph .
BASIC RESEARCH IN CARDIOLOGY, 2011, 106 (06) :1069-1085
[2]   Muscarinic and nicotinic ACh receptor activation differentially mobilize Ca2+ in rat intracardiac ganglion neurons [J].
Beker, F ;
Weber, M ;
Fink, RHA ;
Adams, DJ .
JOURNAL OF NEUROPHYSIOLOGY, 2003, 90 (03) :1956-1964
[3]   CARDIOVASCULAR CONTROL BY CHOLINERGIC MECHANISMS IN THE CENTRAL NERVOUS-SYSTEM [J].
BREZENOFF, HE ;
GIULIANO, R .
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1982, 22 :341-381
[4]  
CERVINI R, 1994, NEUROREPORT, V5, P1346
[5]   Exposure to a hot environment can activate rostral ventrolateral medulla-projecting neurones in the hypothalamic paraventricular nucleus in conscious rats [J].
Cham, Joo Lee ;
Badoer, Emilio .
EXPERIMENTAL PHYSIOLOGY, 2008, 93 (01) :64-74
[6]   Brain Stem NOS and ROS in Neural Mechanisms of Hypertension [J].
Chan, Samuel H. H. ;
Chan, Julie Y. H. .
ANTIOXIDANTS & REDOX SIGNALING, 2014, 20 (01) :146-163
[7]   Interplay between brain stem angiotensins and monocyte chemoattractant protein-1 as a novel mechanism for pressor response after ischemic stroke [J].
Chang, Alice Y. W. ;
Li, Faith C. H. ;
Huang, Chi-Wei ;
Wu, Julie C. C. ;
Dai, Kuang-Yu ;
Chen, Chang-Han ;
Li, Shau-Hsuan ;
Su, Chia-Hao ;
Wu, Re-Wen .
NEUROBIOLOGY OF DISEASE, 2014, 71 :292-304
[8]   High, but not low, exercise volume shifts the balance of renin-angiotensin system toward ACE2/Mas receptor axis in skeletal muscle in obese rats [J].
Dalla Corte Frantz, Eliete ;
Giori, Isabele Gomes ;
Machado, Marcus Vinicius ;
Magliano, D'Angelo Carlo ;
Freitas, Fernanda Marques ;
Boeta Andrade, Mariana Sodre ;
Vieira, Aline Bomfim ;
Lucas Nobrega, Antonio Claudio ;
Tibirica, Eduardo .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2017, 313 (04) :E473-E482
[9]   Maternal protein restriction induced-hypertension is associated to oxidative disruption at transcriptional and functional levels in the medulla oblongata [J].
de Brito Alves, Jose L. ;
de Oliveira, Jessica M. D. ;
Ferreira, Diorginis J. S. ;
Barros, Monique A. de V. ;
Nogueira, Viviane O. ;
Alves, Debora S. ;
Vidal, Hubert ;
Leandro, Carol G. ;
Lagranha, Claudia J. ;
Pirola, Luciano ;
da Costa-Silva, Joao H. .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2016, 43 (12) :1177-1184
[10]   Integrative Physiological Aspects of Brain RAS in Hypertension [J].
de Morais, Sharon D. B. ;
Shanks, Julia ;
Zucker, Irving H. .
CURRENT HYPERTENSION REPORTS, 2018, 20 (02)
1234567