Fractal properties of macrophage membrane studied by AFM

被引：19

作者：

Bitler, A. ^{[1
]}

Dover, R. ^{[2
]}

Shai, Y. ^{[2
]}

机构：

[1] Weizmann Inst Sci, Dept Chem Res Support, Fac Chem, IL-76100 Rehovot, Israel

[2] Weizmann Inst Sci, Fac Biochem, Dept Biol Chem, IL-76100 Rehovot, Israel

来源：

MICRON | 2012年 / 43卷 / 12期

关键词：

Atomic force microscope; Macrophage membrane; Fractal dimension; ATOMIC-FORCE MICROSCOPY; OXIDATIVE STRESS; CELLS; ORGANIZATION; DYNAMICS; CAPTURE; IMAGES; MORPHOLOGY; PROBE;

D O I：

10.1016/j.micron.2012.04.009

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Complexity of cell membrane poses difficulties to quantify corresponding morphology changes during cell proliferation and damage. We suggest using fractal dimension of the cell membrane to quantify its complexity and track changes produced by various treatments. Glutaraldehyde fixed mouse RAW 264.7 macrophage membranes were chosen as model system and imaged in PeakForce QNM (quantitative nanomechanics) mode of AFM (atomic force microscope). The morphology of the membranes was characterized by fractal dimension. The parameter was calculated for set of AFM images by three different methods. The same calculations were done for the AFM images of macrophages treated with colchicine, an inhibitor of the microtubule polymerization, and microtubule stabilizing agent taxol. We conclude that fractal dimension can be additional and useful parameter to characterize the cell membrane complexity and track the morphology changes produced by different treatments. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1239 / 1245

页数：7

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