Detection and characterization of a new betasatellite: variation in disease symptoms of tomato leaf curl Pakistan virus-India due to associated betasatellite

被引：7

作者：

Kumar, Jitendra ^{[1
,2
]}

Singh, Sudhir P. ^{[1
]}

Kumar, Abhinav ^{[2
]}

Khan, Jawaid A. ^{[2
]}

Tuli, Rakesh ^{[1
]}

机构：

[1] Natl Agri Food Biotechnol Inst, Mohali 160071, Punjab, India

[2] Natl Bot Res Inst, Mol Virol Lab, Lucknow 226001, Uttar Pradesh, India

来源：

ARCHIVES OF VIROLOGY | 2013年 / 158卷 / 01期

关键词：

Geminivirus; Begomovirus; Betasatellite; Phylogenetic analysis; Recombination; MOLECULAR CHARACTERIZATION; TOBACCO; IDENTIFICATION; BEGOMOVIRUS;

D O I：

10.1007/s00705-012-1486-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A begomovirus and its associated betasatellites were amplified and sequenced from tobacco plants affected with leaf curl disease. The begomovirus was identified as a new strain of tomato leaf curl Pakistan virus (ToLCPKV), which is referred to here as ToLCPKV-India. A previously known betasatellite [tomato leaf curl Patna betasatellite (ToLCPaB)] and a new betasatellite were also found in leaf-curl-affected samples. The use of infectious clones of ToLCPKV-IN plus ToLCPaB for agroinoculation led to typical leaf curl, while ToLCPKV-IN together with the new betasatellite resulted in curling and chlorosis of leaves. Based on these disease symptoms, we propose to name the new betasatellite tobacco leaf chlorosis betasatellite (TbLChB).

引用

页码：257 / 261

页数：5

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