Wintergreen oil: A novel method in Wheatley's trichrome staining technique

被引：20

作者：

Salleh, Fatmah Md ^{[1
]}

Anuar, Tengku Shahrul ^{[1
]}

Yasin, Azlin Mohd ^{[1
]}

Moktar, Norhayati ^{[1
]}

机构：

[1] Univ Kebangsaan Malaysia, Fac Med, Dept Parasitol & Med Entomol, Kuala Lumpur 50300, Malaysia

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 91卷 / 01期

关键词：

Wintergreen oil; Wheatley's trichrome; Staining technique;

D O I：

10.1016/j.mimet.2012.08.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique, as the reference technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：174 / 178

页数：5

共 8 条

[1]

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