Reduction in energy efficiency induced by expression of the uncoupling protein, UCP1, in mouse liver mitochondria

被引：0
|
作者：
González-Muniesa, P [1 ]
Milagro, FI [1 ]
Campión, J [1 ]
Martínez, JA [1 ]
机构：
[1] Univ Navarra, Dept Physiol & Nutr, Pamplona 31008, Navarra, Spain
来源：
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2006年 / 17卷 / 04期
关键词：
energy metabolism; uncoupling protein-1; gene delivery; hydrodynamics; ATP; UCP;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Uncoupling Protein 1 (UCP1) is an inner mitochondrial membrane protein, uniquely expressed in brown adipocytes, which uncouples the mitochondrial respiration impairing ATP production and energy efficiency. The aim of the present study was to express UCP1 in liver mitochondria using a non-viral system in order to affect energy utilization. The effect of ectopic protein expression on liver energy metabolism, which was evaluated 42 h after DNA transfer, showed that mitochondria expressing UCP1 presented decreased ATP production, lasted more time in membrane potential state 3, and consumed more molecular oxygen to produce the same amount of ATP than the control group. In summary, the successful functionality of the mitochondrial protein, UCP1, after hydrodynamic delivery is a novel and significant finding. This approach could be useful to ectopically express mitochondrial proteins and, in this particular case, to manage metabolic disorders related to energy efficiency and expenditure, such as obesity.
引用
收藏
页码：591 / 597
页数：7
相关论文
共 50 条
  • [21] Regulation of H2O2 generation by uncoupling protein UCP1 in bat and thymus mitochondria
    Dlaskova, Andrea
    Porter, Richard K.
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2008, 1777 : S65 - S65
  • [22] Mechanism of Fatty-Acid-Dependent UCP1 Uncoupling in Brown Fat Mitochondria
    Fedorenko, Andriy
    Lishko, Polina V.
    Kirichok, Yuriy
    CELL, 2012, 151 (02) : 400 - 413
  • [23] Lipolysis and uncoupling protein 1 (UCP1) expression in human visceral adipocytes are induced by nebivolol but not by other beta1 blockers
    Tchaikovski, V.
    Boehmer, F. -D.
    Waltenberger, J.
    EUROPEAN HEART JOURNAL, 2009, 30 : 889 - 889
  • [24] Lipolysis and uncoupling protein 1 (UCP1) expression in human visceral adipocytes are induced by nebivolol but not by other beta1 blockers
    Sarzani, R.
    Bordicchia, M.
    Scappini, L.
    Marcucci, P.
    Siquini, W.
    Fianchini, A.
    Dessi-Fulgheri, P.
    Rappelli, A.
    EUROPEAN HEART JOURNAL, 2009, 30 : 889 - 889
  • [25] Substitutional mutations in the uncoupling protein-specific sequences of mitochondrial uncoupling protein UCP1 lead to the reduction of fatty acid-induced H+ uniport
    Urbánková, E
    Hanák, P
    Skobisová, E
    Ruzicka, M
    Jezek, P
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2003, 35 (02): : 212 - 220
  • [26] The Environmental Pollutants Perfluorooctane Sulfonate and Perfluorooctanoic Acid Upregulate Uncoupling Protein 1 (UCP1) in Brown-Fat Mitochondria Through a UCP1-Dependent Reduction in Food Intake
    Shabalina, Irina G.
    Kramarova, Tatiana V.
    Mattsson, Charlotte L.
    Petrovic, Natasa
    Qazi, Mousumi Rahman
    Csikasz, Robert I.
    Chang, Shu-Ching
    Butenhoff, John
    DePierre, Joseph W.
    Cannon, Barbara
    Nedergaard, Jan
    TOXICOLOGICAL SCIENCES, 2015, 146 (02) : 334 - 343
  • [27] Role of intrahelical arginine residues in functional properties of uncoupling protein (UCP1)
    Echtay, KS
    Bienengraeber, M
    Klingenberg, M
    BIOCHEMISTRY, 2001, 40 (17) : 5243 - 5248
  • [28] The structure and function of the brown fat uncoupling protein UCP1:: current status
    Rial, E
    González-Barroso, MM
    Fleury, C
    Bouillaud, F
    BIOFACTORS, 1998, 8 (3-4) : 209 - 219
  • [29] A role for ubiquitinylation and the cytosolic proteasome in turnover of mitochondrial uncoupling protein 1 (UCP1)
    Clarke, Kieran J.
    Adams, Alison E.
    Manzke, Lars H.
    Pearson, Terry W.
    Borchers, Christoph H.
    Porter, Richard K.
    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 2012, 1817 (10): : 1759 - 1767
  • [30] UCP1: The Original Uncoupling Protein—and Perhaps the Only One? New Perspectives on UCP1, UCP2, and UCP3 in the Light of the Bioenergetics of the UCP1-Ablated Mice
    Jan Nedergaard
    Anita Matthias
    Valeria Golozoubova
    Anders Jacobsson
    Barbara Cannon
    Journal of Bioenergetics and Biomembranes, 1999, 31 : 475 - 491
12345