Mining of EST-SSR from 454 pyrosequencing in the surf clam Mesodesma donacium (Lamark, 1818)

被引：6

作者：

Aguilar-Espinoza, A. ^{[1
]}

Guzman-Riffo, B. ^{[1
]}

Haye, P. A. ^{[2
,3
]}

Gallardo-Escarate, C. ^{[1
]}

机构：

[1] Univ Concepcion, Ctr Biotecnol, Dept Oceanog, Lab Biotecnol & Genom Acuicola, Concepcion, Chile

[2] Univ Catolica Norte, Fac Ciencias Mar, Dept Biol Marina, Lab Diversidad Mol, Coquimbo, Chile

[3] Ctr Estudios Avanzados Zonas Aridas, Coquimbo, Chile

来源：

CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 04期

关键词：

Pyrosequencing; 454; Expressed sequence tag (EST); Microsatellite; Mesodesma donacium; MICROSATELLITE MARKERS; UNIVERSAL; PROGRAM;

D O I：

10.1007/s12686-012-9652-3

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Novel simple sequence repeats (SSR) were identified from 454 transcriptome pyrosequencing ESTs in the surf clam Mesodesma donacium. From 7,734 ESTs annotated with SSR motives, 75 putative EST-SSRs were selected according their repetitive DNA sequence and functional annotation (Gene ontology), of these, 15 were characterized on 45 individuals of two wild populations located in two different biogeographic transition zones of the coast of Chile (30 and 42A degrees S). Most loci were in Hardy-Weinberg equilibrium, and the polymorphic information content (PIC) ranged from 0.243 to 0.634. Pairwise F (st) between sampled populations was estimated in 0.478. These EST-SSR markers promise to be useful for future management strategies of this overexploited species.

引用

页码：829 / 832

页数：4

共 19 条

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